We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.


Titanium Micro-Spikes Skewer Resistant Superbugs

Ruptured Candida cell on top of titanium micro-spikes.
A ruptured Candida cell on top of the titanium micro-spikes, magnified 25,000 times. Credit: RMIT University
Listen with
Register for free to listen to this article
Thank you. Listen to this article using the player above.

Want to listen to this article for FREE?

Complete the form below to unlock access to ALL audio articles.

Read time: 2 minutes

A new study suggests rough surfaces inspired by the bacteria-killing spikes on insect wings may be more effective at combatting drug-resistant superbugs, including fungus, than previously understood.

The increasing rates of drug-resistant infection has health experts globally concerned.

To avoid infection around implants – such as titanium hips or dental prosthesis – doctors use a range of antimicrobial coatings, chemicals and antibiotics, but these fail to stop antibiotic-resistant strains and can even increase resistance.

To address these challenges, RMIT University scientists have designed a pattern of microscale spikes that can be etched onto titanium implants or other surfaces to provide effective, drug-free protection from both bacteria and fungus.

Want more breaking news?

Subscribe to Technology Networks’ daily newsletter, delivering breaking science news straight to your inbox every day.

Subscribe for FREE

The team’s study published in Advanced Materials Interfaces tested the effectiveness of the altered titanium surface in killing multi drug-resistant Candida – a potentially deadly fungus responsible for one in 10 hospital-acquired medical device infections.

The specially designed spikes, each of a similar height to a bacteria cell, destroyed about half the cells soon after contact.

Significantly, the other half not immediately destroyed were rendered unviable from the injuries sustained, unable to reproduce or cause infection.

Lead Postdoctoral researcher, Dr Denver Linklater, said metabolic analysis of protein activity revealed both the Candida albicans and multi-drug resistant Candida auris fungi cells sitting injured on the surface were as good as dead.

“The Candida cells that were injured underwent extensive metabolic stress, preventing the process where they reproduce to create a deadly fungal biofilm, even after seven days,” said Linklater, from RMIT’s School of Science.

“They were unable to be revived in a non-stress environment and eventually shut down in a process known as apoptosis, or programmed cell death.”

The surface's effectiveness against common pathogenic bacteria including golden staph was demonstrated in an earlier study published in Materialia.

Group leader, Distinguished Professor Elena Ivanova, said the latest findings shed light on the design of antifungal surfaces to prevent biofilm formation by dangerous, multi-drug resistant yeasts.

“The fact that cells died after initial contact with the surface – some by being ruptured and others by programmed cell death soon after – suggests that resistance to these surfaces will not be developed,” she said.

“This is a significant finding and also suggests that the way we measure the effectiveness of antimicrobial surfaces may need to be rethought.”

Advances have been made over the past decade in designing surfaces that kill superbugs on contact. However, finding the right types of surface patterns to eliminate 100% of microbes so some don’t survive to become resistant is an ongoing challenge.

“This latest study suggests that it may not be entirely necessary for all surfaces to eliminate all pathogens immediately upon contact if we can show that the surfaces are causing programmed cell death in the surviving cells, meaning they die regardless,” she said.

Leading the way in bioinspired solutions

RMIT’s Multifunctional Mechano-biocidal Materials Research Group has led the world for over a decade in the development of antimicrobial surfaces inspired by the nanopillars covering dragonfly and cicada wings.

Ivanova herself was among the first to observe how when bacteria settle on an insect wing, the pattern of nanopillars pulls the cells apart, fatally rupturing the membranes.

“It’s like stretching a latex glove,” Ivanova said. “As it slowly stretches, the weakest point in the latex will become thinner and eventually tear.”

Her team have spent the past decade replicating these insects’ nanopillars in nanopatterns of their own, with this latest advance achieved using a technique called plasma etching to create the antibacterial and antifungal pattern in titanium.

Ivanova said the relatively simple etching technique could be optimised and applied to a wide range of materials and applications.

“This new surface modification technique could have potential applications in medical devices but could also be easily tweaked for dental applications or for other materials like stainless steel benches used in food production and agriculture,” she said.

Reference: Le PH, Linklater DP, Aburto-Medina A, et al. Apoptosis of multi-drug resistant Candida species on microstructured titanium surfaces. Adv Mater Interfaces. 2023:2300314. doi: 10.1002/admi.202300314

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.