A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial Autosampler
Poster Jul 06, 2017
Christian Berchtold, Guenter Boehm, Renée Falsia, Thomas Preiswerk, Götz Schlotterbeck
- The new LCMS-P 80 Tool is capable of pipetting whole blood with normal hematocrit levels (30-50%) directly from primary tubes with good accuracy and precision, applying one average correction factor. For high hematocrit levels a different correction factor should be applied.
- Wash procedures were established to minimize carryover of analytes. A procedure to minimize the deposition of blood on system surfaces proofed successful.
- The system consisting of a commercial PAL RTC autosampler, connected to an Agilent LC-MS system can perform the complete sample prep and LC-MS analysis for whole blood samples from primary tube. The performance of the system was tested with several typical compounds in therapeutically relevant concentrations.
- Future work is aimed at evaluating the capability of the sensor to reliably detect certain error conditions.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE