A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial Autosampler
Poster Jul 06, 2017
Christian Berchtold, Guenter Boehm, Renée Falsia, Thomas Preiswerk, Götz Schlotterbeck
- The new LCMS-P 80 Tool is capable of pipetting whole blood with normal hematocrit levels (30-50%) directly from primary tubes with good accuracy and precision, applying one average correction factor. For high hematocrit levels a different correction factor should be applied.
- Wash procedures were established to minimize carryover of analytes. A procedure to minimize the deposition of blood on system surfaces proofed successful.
- The system consisting of a commercial PAL RTC autosampler, connected to an Agilent LC-MS system can perform the complete sample prep and LC-MS analysis for whole blood samples from primary tube. The performance of the system was tested with several typical compounds in therapeutically relevant concentrations.
- Future work is aimed at evaluating the capability of the sensor to reliably detect certain error conditions.
It has become increasingly important for chemists and analysts to be able to test and determine the various components that may be present in mixtures, to solve a wide variety of scientific problems. NMR spectroscopy has emerged as a powerful, non-destructive analytical technique for this.READ MORE
Biologics-based medicines is a rapidly growing field and a promising solution to address many unmet medical needs. Biopharmaceutical developers are actively looking for more efficient and robust production platforms to reduce timelines and overall development costs. Gibco™ Expi™ Expression Systems are complete platforms that can speed up biologics development.READ MORE