CAPILLARY ELECTROPHORESIS OF APIGENIN
Poster Aug 25, 2014
Paulina Furmaniak, Pawel Kubalczyk, Rafal Glowacki
Increasing interest in phytocompounds especially flavonoids is a result of their widespread occurrence in plant kingdom and wide range of biological activity. Apigenin (4,5,7-trihydroxyflavone), a naturally occurring plant flavone is a potent antioxidant that exhibits anti-inflammatory activities, is a scavenger of free radicals, and also prevents oxidation of vitamins C, E and glutathione. Apigenin exhibit some antitumor effects by inhibiting the tyrosine kinase activity of topoisomerase, angiogenesis and protecting against oxidative damage of DNA.
Capillary electrophoresis (CE) is a modern analytical technique which allows effective separation of charged particles in electric field. CE is considered as a green analytical technique due to its low reagent consumption, small sample volume and short analysis time. Unfortunately, important limitation on the application of capillary electrophoresis to biological samples is high limit of detection.
Very important role in the process of increasing the sensitivity of capillary electrophoresis play an analyte sweeping methods inside the capillary. One promising modality for sweeping the sample in the capillary is micellar electrokinetic chromatography (MEKC). The most suitable electrolyte for this separation was a buffer solution (pH 10.1) of 0.03 M sodium borate with addition of sodium dodecyl sulfate (0.01M) and acetonitrile (10%). Analysis of a complex matrix (plant extracts) requires precise step of sample preparation. The air-dried parsley leaves were powdered and several times extracted with methanol and ethyl acetate. The extract was in the last step centrifuged and the resulting supernatant was subjected to CE analysis. All separations were performed at 25C with detector set to measure peaks at 390 nm.
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.
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9th Edition of International Conference on Analytical Chemistry
Mar 19 - Mar 20, 2018