CAPILLARY ELECTROPHORESIS OF APIGENIN
Increasing interest in phytocompounds especially flavonoids is a result of their widespread occurrence in plant kingdom and wide range of biological activity. Apigenin (4,5,7-trihydroxyflavone), a naturally occurring plant flavone is a potent antioxidant that exhibits anti-inflammatory activities, is a scavenger of free radicals, and also prevents oxidation of vitamins C, E and glutathione. Apigenin exhibit some antitumor effects by inhibiting the tyrosine kinase activity of topoisomerase, angiogenesis and protecting against oxidative damage of DNA[1].
Capillary electrophoresis (CE) is a modern analytical technique which allows effective separation of charged particles in electric field. CE is considered as a green analytical technique due to its low reagent consumption, small sample volume and short analysis time[2]. Unfortunately, important limitation on the application of capillary electrophoresis to biological samples is high limit of detection.
Very important role in the process of increasing the sensitivity of capillary electrophoresis play an analyte sweeping methods inside the capillary. One promising modality for sweeping the sample in the capillary is micellar electrokinetic chromatography (MEKC). The most suitable electrolyte for this separation was a buffer solution (pH 10.1) of 0.03 M sodium borate with addition of sodium dodecyl sulfate (0.01M) and acetonitrile (10%). Analysis of a complex matrix (plant extracts) requires precise step of sample preparation. The air-dried parsley leaves were powdered and several times extracted with methanol and ethyl acetate. The extract was in the last step centrifuged and the resulting supernatant was subjected to CE analysis. All separations were performed at 25C with detector set to measure peaks at 390 nm.
Capillary electrophoresis (CE) is a modern analytical technique which allows effective separation of charged particles in electric field. CE is considered as a green analytical technique due to its low reagent consumption, small sample volume and short analysis time[2]. Unfortunately, important limitation on the application of capillary electrophoresis to biological samples is high limit of detection.
Very important role in the process of increasing the sensitivity of capillary electrophoresis play an analyte sweeping methods inside the capillary. One promising modality for sweeping the sample in the capillary is micellar electrokinetic chromatography (MEKC). The most suitable electrolyte for this separation was a buffer solution (pH 10.1) of 0.03 M sodium borate with addition of sodium dodecyl sulfate (0.01M) and acetonitrile (10%). Analysis of a complex matrix (plant extracts) requires precise step of sample preparation. The air-dried parsley leaves were powdered and several times extracted with methanol and ethyl acetate. The extract was in the last step centrifuged and the resulting supernatant was subjected to CE analysis. All separations were performed at 25C with detector set to measure peaks at 390 nm.
