Data Management System for Distributed Virtual Screening
Poster Mar 04, 2009
To manage HTD data effectively and efficiently, we have developed a distributed virtual screening data manage- ment system (DVSDMS) by integrating the open source database MySQL into high throughput docking.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE