Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)
Poster Apr 24, 2017
Reto Bolliger, Hans-Joachim Huebschmann, Guenter Boehm
The composition of the fat in chocolate is an important factor influencing the quality of chocolate. Specifically the replacement of cocoa butter by replacements or CBEs (cocoa butter equivalents) is of interest to food quality laboratories .
The determination of fat content and composition of food samples via fatty acid methyl esters is a very common task in governmental, quality control (QC) or contract research laboratories (CRO).
This work presents a fully automated workflow of this procedure using an autosampler with robotic tool change (PAL RTC). The automated workflow improves process safety and minimizes handling errors. The PAL RTC was equipped with (i) an automated dilutor to handle all liquids for the reactions and the extraction and for the cleaning steps, a 25 µL syringe for internal standard addition, (ii) a vortex module to provide fast mixing and extracting and (iii) a normal 10µl syringe tool to inject the sample to the GC. The software of the sampler allows overlapped (prep ahead) sample processing, which increases the efficiency and sample throughput.
The method allows the determination of total fat content, quantitative analysis of saturated and unsaturated cis- and trans- fatty acids. The use of three internal standards allows controlling the yield of extraction, transesterification and undesired saponification [2-4]. Chocolate samples from Japan, Switzerland and the USA were analyzed. The data allowed a clear classification of the chocolates in terms of content of cocoa butter and replacements.
 Buchgraber M, Andronis S, J. AOAC 90 (2007) 1326-1339
 Arens M. et al., Fat Sci. Technol. 96 (1994) 67-68
 Suter B. et al., Z Lebensm. Unters. Forsch. A 204 (1997) 252-258
 de Koning S. et al., J. Chromatography A 922 (2001) 391–397
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Comments | 0 ADD COMMENT
2nd Annual Artificial Intelligence in Drug Development Congress
Sep 20 - Sep 21, 2018