We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement

Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA

Experimental design considerations for efficient and specific gene  knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA content piece image
The bacterial CRISPR-Cas9 system has revolutionized the genome engineering world with its efficiency and ease of use. The most common use of the CRISPR-Cas9 technology has been to engender gene knockouts that are generated as a result of imperfect repair of a targeted double-strand DNA break by the non-homologous end joining (NHEJ) pathway.