Gene Silencing Approaches for Functional Angio-Genomics
Introduction
This project focuses on bypassing difficulties and disadvantages of classical homologous recombination to generate animal models with custom genetic alterations, by developing alternative novel and promising approaches for semi-high throughput gene targeting or gene silencing in vivo. We have optimized procedures for construct design, transfection, gene delivery and genotyping. We are evaluating these novel strategies to study genes involved in angiogenesis.
This project focuses on bypassing difficulties and disadvantages of classical homologous recombination to generate animal models with custom genetic alterations, by developing alternative novel and promising approaches for semi-high throughput gene targeting or gene silencing in vivo. We have optimized procedures for construct design, transfection, gene delivery and genotyping. We are evaluating these novel strategies to study genes involved in angiogenesis.
