High-Throughput RNAi by Reverse Transfection with low siRNA Concentrations
Introduction
The use of short interfering RNA (siRNA) for knockdown of gene expression has become a powerful tool in molecular and cell biology. Some applications require the use of low siRNA concentrations (less than 5 nM), for example, to decrease the possibility of nonspecific effects. We present data from experiments carried out using a new transfection reagent, HiPerFect Transfection Reagent, which allows efficient gene knockdown with £nM siRNA.
The use of short interfering RNA (siRNA) for knockdown of gene expression has become a powerful tool in molecular and cell biology. Some applications require the use of low siRNA concentrations (less than 5 nM), for example, to decrease the possibility of nonspecific effects. We present data from experiments carried out using a new transfection reagent, HiPerFect Transfection Reagent, which allows efficient gene knockdown with £nM siRNA.
