INTERACTION BETWEEN WHEY PROTEIN NANOPARTICLES AND FATTY ACIDS
Poster Aug 18, 2014
Hassan, Z.M.R*, Awad R. A.*, El-Sayed, M. M. **, Mevat I. Foda**, Otzen, D.*** and Heba H. Salama**
The cytotoxicity of formulated nanoparticle complexes of different fatty acids (oleic, eliedic, Cis-vaccenic, Trans-vaccenic, and linolenic acids) in the presence or absence of whey protein isolate (WPI) was investigated in this study. Nanoparticle complexes formed with WPI was examined for surface tension, circular dichroism (CD), turbidity, isothermal titration calorimetry (ITC) and Cytotoxic activity. Surface tension values were decreased with adding fatty acid to WPI. Cis-fatty acids such as oleic, cis-vaccenic and linolenic caused higher decrease in the surface tension of WPI nanoparticles than that of trans-fatty acids (eledic and trans-vaccenic acids). The tertiary structure of WPI was lost and changed from fold to unfold after binding with fatty acids. The changes in WPI structure would be correlated to exhibit a cytotoxic activity to tumor cells. The turbidity values for nanocomplexes of WPI/fatty acids were lower confirming higher ability in binding fatty acids. All nano complexes formed of WPI/fatty acids exhibited a cytotoxic ability as a lysis in erythrocytes. Nanocomplexes can be formed from WPI with good cytotoxic effect to tumer cells using cis-vaccenic and linolenic fatty acids comparable to oleic acid. It was a new interesting observation being that the nanocomplexes formed of WPI with fatty acids has a comparable cytotoxcisty to that of α-LA and β-lg and can be used in tumor therapy.
Keywords: Nanoparticles; Whey protein isolate; Fatty acids; Surface tension; Circular dichroism; Turbidity; Cytotoxicity.
High Throughput Rheological Characterization of Small Volume Biopharmaceutical FormulationsPoster
Importance of assessing viscosity fingerprints of small molecules.READ MORE
Optimization of a Vacuum Ultraviolet Photoionization source for GC used with a High Resolution TOFMSPoster
-Tune solution allows optimization of ion source parameters for
both proton transfer and direct ionization
-Independent ionization processes exist for M+ and MH+
-Optimizing for dopant signal intensity yields inferior results
-Degree of fragmentation remains relatively constant over a
range of source conditions
The Use of GC/MS to Identify Contamination, Counterfeiting and Adulteration of SpicesPoster
Our previous study of spices was designed to evaluate the metal content in the spices for evidence of contamination by heavy metals or for adulteration and counterfeiting by notable concentrations of wear or additive metals. This study targeted the same spice samples to quantify the characteristic primary and secondary market compounds for each spice group.READ MORE