LC/MS/MS Quantitation of Eicosapentaenoic Acid in Rat Plasma
Poster Jun 19, 2013
Rachel Sun, Jordan Nally, Tim Shoaf
Since EPA appears in many different forms, it will need to be released from conjugated forms by hydrolysis in order to quantitate total EPA in plasma samples. Plasma samples were first incubated under acidic conditions (ACN/6N HCl) for 1 hour at 100C to release EPA, liquid-liquid extraction with hexanne followed to clean up the samples from the matrix. EPA-d5 was added as an internal standard before hydrolysis. Hexand supernatant was evaporated to dryness, reconstittued with ACN/water and injected into an LC/MS/MS using a Kinetex XB-C18 column with an ammonium acetate/ACN/H20 mobile phase. TSQ quantum instrument at negative mode was used for detection/quantitation.
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolismPoster
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.READ MORE
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.
Multi-Residue Pesticide Analysis in Food Matrices Using Ultivo Triple Quad LC/MSPoster
Regulatory agencies have set maximum residue levels for hundreds of pesticides and their metabolites in foods.READ MORE
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