Monitoring Protein Synthesis in Living Cells With Fluorescent Labeled tRNA FRET Pairs
CHO cells were co-transfected with bulk Cy3- and Rho110- labeled yeast tRNAs. At 7h post transfection, cells were treated or not with puromycin or cycloheximide prior to fixation and imaging. CHO cells, infected with EHDV2-IBAV were co-transfected with Cy3 and Rho110 fl-tRNA. Results show typical triple co-localizations, consistent with the formation of ‘viral factories’.
