Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent Model
Poster Nov 21, 2017
J. R. A. Newton, N. Couto, J. Barber, R. Bojar, J. Sidaway and M. Clench
Current animal-free approaches for assessing the metabolism of xenobiotics in skin are limited. The primary reason for this is that the levels and distribution of xenobiotic metabolising enzymes in skin are not well characterised. The overall aim of this project was to develop a combined in silico-in vitro system for predicting xenobiotic metabolism in human skin from experiments carried out on a living skin equivalent (LSE). In order to do this it was required to systematically quantify the level of xenobiotic metabolising enzymes in skin and in Labskin using proteomics.
In order to examine the distribution of enzymes detected, a novel substrate-based mass spectrometry imaging technique was proposed that would allow the activity of xenobiotic metabolising enzymes to be localised within the in vitro model. Ultimately the project will use these proteomic and imaging data to build a pharmacokinetic (PBPK) model of human skin xenobiotic metabolism and disposition to enable in vitro to in vivo extrapolation (IVIVE) of LSE based xenobiotic metabolism data. This integrated solution would then offer an animal-free alternative that will increase screening capacity and knowledge of xenobiotic skin metabolism.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE
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2nd Annual Artificial Intelligence in Drug Development Congress
Sep 20 - Sep 21, 2018