Bioo Scientific Launches a Sensitive ELISA for the Detection of Amantadine Residues in Meat

Amantadine (Symmetrel) is a drug that was approved by the US Food and Drug Administration for use as an antiviral and an antiparkinsonian drug, and has been used by Chinese poultry farmers to protect birds against avian influenza. However, the safety and efficacy of amantadine have been challenged, and its use as a veterinary drug is now banned in China. However, it has been reported by numerous sources that some poultry farmers continue to give chickens amantadine to help them survive in overcrowded chicken houses.

The sensitive MaxSignal Amantadine ELISA Test Kit, with detection limits of 0.25 ppb in meat, is based on a competitive colorimetric ELISA assay. Amantadine residue present in the sample will compete for HRP-conjugated antibodies against amantadine, thereby preventing the Amantadine-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the concentration of amantadine residue in the sample.

The MaxSignal Amantadine ELISA Test Kit joins Bioo Scientific’s premier line of ELISAs for the detection of veterinary drugs in food and feed samples. These kits are all manufactured to the international quality standard ISO 9001:2008 (ISO CI#: SARA-2009-CA-0114-02-A).