Breaking Barriers in siRNA Delivery
Product News Dec 17, 2014
To accelerate the understanding of disease and the development of gene knockdown therapies, Integrated DNA Technologies (IDT) is facilitating the advancement of a new transfection technique. This nanoparticle-based method is explored in a recent DECODED article contributed by Precision NanoSystems Inc. (PNI).
By combining IDT’s highly potent Dicer-substrate RNAs (DsiRNAs) and PNI’s unique nanoparticle delivery system for hard-to-transfect cells, the IDT-PNI partnership presents a successful solution for challenging gene silencing applications, achieving >95% transfection efficiency.
The novel NanoAssemblr™ system and SUB9KITS™ reagent kits from PNI enable the simple assembly of novel nanoparticles using advanced microfluidics, which minimizes waste material and ensures a consistently tuned particle size. These particles are designed to deliver DsiRNA into the cell via a natural pathway, avoiding carrier effects and limitations, such as toxicity and poor performance in postmitotic cells. And the particles greatly enhance the efficiency of RNA transfection, even in notoriously difficult-to-transfect neurons.
Scientists can now focus on studying the effects of gene knockdown, enabling many new and exciting research projects that were previously limited by the low transfection efficiency and/or high toxicity of traditional delivery methods.