Troubleshooting qPCR - Integrated DNA Technologies to Host Webinar
Dr Aurita Menezes, who has a PhD in microbiology, will provide a brief overview of quantitative polymerase chain reaction (qPCR) and why it is the method of choice for accurately quantitating gene expression. However, when reactions do not proceed as expected, troubleshooting can prove challenging.
There are numerous factors that can affect the efficiency of amplification and thus the integrity of resulting data, including sample quality, template quantity, mastermix differences, assay design and primer/probe re-suspensions. During this webinar, Dr Menezes will cover a variety of potential issues that can affect any qPCR protocol, providing details of how they are manifested within the amplification curve, and the best techniques to overcome such concerns. Register now to attend the webinar in your time zone.
IDT offers a range of informative and educational webinars, all of which are hosted online. Check them out in IDT’s tech vault. For further information about IDT and its portfolio of primers, probes and PrimeTime qPCR assays, visit www.idtdna.com. For real-time updates, follow us on Twitter @idtdna.