CellScape™ Precise Spatial Proteomics Platform

Name: CellScape™ Precise Spatial Proteomics Platform
Brand: Bruker

Credit: Bruker

The CellScape™ Precise Spatial Proteomics platform is a cutting-edge imaging solution for quantitative in situ spatial phenotyping. The CellScape platform enables highly multiplexed spatial proteomics with single-cell resolution for whole-slide analyses of tumor microenvironments as well as deep immune profiling for applications in immunology, neuroscience, and infectious disease.

The CellScape instrument features market-leading optical performance, integrated fluidics for walk-away automation, and unprecedented flexibility in assay design. Supported by high dynamic range imaging, the CellScape platform can detect both high- and low-expressing biomarker targets simultaneously. Sample preparation is simple: the CellScape Whole-Slide Imaging Chamber converts a standard microscope slide into a microfluidic device for staining, imaging, and safe sample storage. The CellScape staining and imaging workflow is powered by EpicIF™ technology for gentle signal removal from standard fluorescently labeled antibodies, circumventing the need for proprietary antibody conjugation and complex assay validation. Jumpstart assay design with VistaPlex™ Multiplex Assay Kits, pre-validated antibody panels that can be combined or expanded to easily create customized assays.

This powerful combination of features and versatility significantly improves spatial biology workflows, accelerating discovery and driving broader adoption of high-plex spatial omics for translational and clinical applications.

Product Specifications

Automation Autonomous image acquisition Autonomous sample staining & plex-iteration Autonomous coordination of multiple experiments Feasible Staining of 15 x 5 markers without Intervention

Throughput Four chip/sample parallel processing Greater than 320 mm2 available surface area per sample 1, 2, 3, or 4 parallel independent protocols Hot-swappable reagents for unlimited plex High-speed, encoded, XY,Z stages (sample/time experiment-dependent)

Optical Performance 20X Plan Apo Lambda D | 0.80 NA sub 405 nm to supra 750 nm chromatic correction 25mm+ planarity, relative to field number In-system registration correction (physical & digital)

Sample Illumination, Excitation Zero autofluor, monochromatic transmitted illumination Pan-spectral (inc. UV), high power arc lamp fluorescent excitation Five independent, zero required compensation imaging channels Target spectra: brilliant UV, brilliant V, FITC, PE, PerCP 5.5 Optimized, spectrally targeted, photo-inactivation channel

Digital Sampling 0.796 mm2 per image at 182 nm/pixel 2.714 mm2 per image at 365 nm/pixel (FalconFAST™ upgrade) High dynamic range image pipeline, 32-bit encoding luminance Transmitted, autofluorescence, & net fluorescence for all acquisitions

Image Analysis In system complete cytometry pipeline Automatic cell segmentation, automatic true NET FLUOR calculation Bivariate gating, gate-parent gate relational statistics, cell call - image linkage Imaging data compatibility with OME standards Cytometry data compatibility with FCS standards

Form Factor & Utility Requirements Compact benchtop scanner with compact benchtop fluidics unit In-built vibration isolation, VIT not required 120 V AC power

About Bruker

Bruker Daltonics is enabling scientists to make breakthrough discoveries and develop new applications that improve the quality of human life. Their high-performance scientific instruments and high-value analytical and diagnostic solutions enable scientists to explore life and materials at molecular, cellular and microscopic levels.

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