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ioGABAergic Neurons – iPSC-Derived Inhibitory Neurons With >95% Purity

ioGABAergic Neurons – iPSC-Derived Inhibitory Neurons With >95% Purity


ioGABAergic Neurons are human induced pluripotent stem cell (iPSC)-derived inhibitory GABAergic neurons, precision reprogrammed using opti-ox™technology, that express classical marker genes including GAD1, GAD2, VGAT, DLX1 and DLX2. These cells provide an easy to use, high-quality, co-culture compatible human model for the study of neurological circuitry, neurodevelopmental (autism, epilepsy) and neurodegenerative (Alzheimer’s) disease.

Using a simple 2-step protocol, consistently go from cryopreserved cells to mature, functional, experiment-ready GABAergic neurons with over 95% purity in just 12 days, as confirmed by single cell RNA sequencing. The high purity of ioGABAergic Neurons solves the problem of heterogeneous neuronal populations, obtained with traditional directed differentiation protocols, where excitatory neurons often obscure inhibitory signals in cell-based assays.

Obtain reproducible results from your assays, regardless of the lot, assay, user, or lab, and save valuable time on batch validation. No prior experience of culturing iPSC-derived cells is required as every vial of ioGABAergic Neurons will consistently generate a highly defined cell population, as confirmed by bulk RNA sequencing.

Calcium imaging has been used to demonstrate that ioGABAergic Neurons display spontaneous activity.  In the human brain, the functions of GABAergic neurons are intimately connected with glutamatergic neurons and astrocytes, making co- or tri-culturing these cell types desirable in vitro. ioGABAergic Neurons are suitable for co- and tri-culture studies with astrocytes and glutamatergic neurons, to more closely mirror and study in vivo brain function.

Fill out the form to speak to an expert about ioGABAergic Neurons. 


Product Specifications
Starting material Human iPSC line
Donor Caucasian adult male (skin fibroblast)
Karyotype Normal (46, XY)
Differentiation method opti-ox cellular reprogramming
Format Cryopreserved cells
Quality control Sterility, protein expression (ICC) and gene expression (RT-qPCR)
Vial size >3 x 10⁶ viable cells
Seeding compatibility 6, 12, 24 & 96 well plates
Recommended seeding density 150,000 cells/cm²
Product use Research use only
About bit.bio
bit.bio is an award-winning human synthetic biology company based in Cambridge, UK. Our cell identity coding platform has the ability to create any human cell type and to manufacture it at scale with precision and consistency. Having access to human cells has the potential to unlock a new generation of medicine: it will enable research and drug discovery to move on from inappropriate models and work with the cells that actually are affected by human disease and form the basis for a new generation of cell and tissue therapies. It will enable us to realise our vision: biomedical innovation and a new generation of cures through precision reprogrammed human cells. The cell identity coding platform consists of three core parts: our discovery platform which identifies the precise code that defines cell fate; our precision cellular reprogramming technology - opti-ox - that deterministically reprograms stem cells into any cell type; and our world leading expertise in stem cell reprogramming, manufacturing and clinical translation. Using our platform, we have already launched multiple wild type and disease model cells under our ioCells™ brand for research and drug discovery. The platform also means we can offer custom partnership capabilities. And we are currently building our own, in-house cell therapy pipeline using our platform.