Anticancer Therapies – Multimedia

Despite extensive research effort there are no accepted clinical biomarkers that predict for response to systemic combination chemotherapy in advanced CRC.

The High Throughput Biomedicine unit at FIMM Technology Centre provides a wide range of biomedical high throughput assays.

Asier Unciti-Broceta, University of Edinburgh, speaking at Discovery Chemistry Congress 2015.

The effect from the c-Mycinhibitor 100258-F4 was clearly observed from the G0/G1 cell cycle arrest and the increase in early and late apoptotic cell. The Cellometer method can measure fluorescent cell-based assays such as cell cycle, apoptosis, protein expression, autophagy and viability. The ability to export the data to FCS Express facilitates simple data analysis and reporting to generate results for publications.

• Celigo Imaging Cytometer is a versatile and powerful tool for 3D tumorspheroid analysis
• Assays such as measuring optimal seeding density for forming tumorspheroids and viability measurement have been demonstrated
• Advanced assays such size measurement, growth inhibition, invasion into matrigel®, migration on to collagen and HUVEC, and tissue invasion that have been performed by manual microscope observation can now be easily quantified using the automated imaging cytometry method

Claire Morgan discusses the need to identify a panel of biomarkers that may distinguish indolent cancer from aggressive metastatic disease when used collectively.

Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.

This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.

An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.

3D Tumorspheroid models for improved predictivity in cancer drug discovery.