CRISPR and Genome Editing – Multimedia

Poster describing the advantages of a DNA-free gene editing system and the application of this system in zebrafish.

An overview of our rational design algorithm for picking highly functional crRNA sequences in combination with comprehensive specificity analysis.

Pathophysiological conditions, medical interventions, and off-target toxicities can all result in cellular oxidative stress. In cardiac myocytes, prolonged and/or excessive oxidative stress can lead to cardiotoxicity: a primary cause of developmental delays, black-box warnings, and post-launch withdrawal of pharmaceuticals.

The CRISPR-Cas9 system has the potential to significantly advance basic and applied research.

Here we describe the strategy for scaffold design, the importance of an optimal promoter, and demonstrate gene target down-regulation from the over-expression of lentiviral microRNA mimics.

Here we demonstrate how to perform lipid based transfections for homology directed repair using DharmaFECT Duo, CRISPR-Cas9 reagents and, synthetic DNA donor oligos.

Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons.

Overview of various CRISPR-Cas9 reagents to provide the highest efficiency of gene editing in your experiments.

We demonstrate that plant improvement by means of transgenesis causse alterations in the expression profiles of endogenous non-target genes.

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.