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CRISPR and Genome Editing – Multimedia

Knockdown of p53 by Accell Self-delivering siRNA Causes Inhibition of p53-dependent DNA Damage Response in IMR-32 Neuroblastoma Cell Line and β-amyloid Toxicity in Rat Cortical Neurons  content piece image
Poster

Knockdown of p53 by Accell Self-delivering siRNA Causes Inhibition of p53-dependent DNA Damage Response in IMR-32 Neuroblastoma Cell Line and β-amyloid Toxicity in Rat Cortical Neurons

Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons.
Increasing Gene Editing Efficiencies in Eukaryotic Cell Lines by Selection of Appropriate CRISPR-Cas9 Reagents  content piece image
Poster

Increasing Gene Editing Efficiencies in Eukaryotic Cell Lines by Selection of Appropriate CRISPR-Cas9 Reagents

Overview of various CRISPR-Cas9 reagents to provide the highest efficiency of gene editing in your experiments.
Impact of the transgene MON 810 in expression of coding and non-coding RNAs in maize content piece image
Poster

Impact of the transgene MON 810 in expression of coding and non-coding RNAs in maize

We demonstrate that plant improvement by means of transgenesis causse alterations in the expression profiles of endogenous non-target genes.
Innovative Technology that Enables RNAi in Difficult to Transfect Cells content piece image
Poster

Innovative Technology that Enables RNAi in Difficult to Transfect Cells

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
Innovative Technology that Enables RNAi in Difficult to Transfect Cells content piece image
Poster

Innovative Technology that Enables RNAi in Difficult to Transfect Cells

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
Video

Capturing D Genome Variation for Wheat Improvement

The diploid D genome species, Aegilops tauschii Coss. is source of allelic variation for hexaploid wheat, Triticum aestivum L and has provided numerous genes for resistance to diseases and insect pests.
Cas9 Driven by an Optimal Promoter Improves Gene Editing in Eukaryotic Cell Lines when Paired with Synthetic crRNA and tracrRNA content piece image
Poster

Cas9 Driven by an Optimal Promoter Improves Gene Editing in Eukaryotic Cell Lines when Paired with Synthetic crRNA and tracrRNA

Presented here are results on the efficiency of using synthetic crRNA and tracrRNA to introduce gene editing events when co-transfected with a plasmid expressing Cas9. We explored the use of antibiotic and fluorescence activated cell sorting (FACS) methods for enrichment of cells that have undergone gene editing, and the use of multiple promoters to increase efficiency of gene editing with Cas9 and synthetic tracrRNA and crRNAs.
Video

A Novel Vertically-Integrated Platform for the Discovery and Molecular Characterization of Lung Cancer Stem Cells

Raj Batra, University of California Los Angeles, speaking at Diagnostics & Biomarkers 2015.
Geneious R8: A Powerful and Comprehensive Suite of Molecular Biology Tools
 content piece image
Poster

Geneious R8: A Powerful and Comprehensive Suite of Molecular Biology Tools


Geneious R8: A powerful and comprehensive suite of molecular biology tools.
Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis content piece image
Poster

Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis

The research reviews current knowledge of the genetic causes of Leber's congenital amaurosis (LCA) and the development of a gene therapy intervention for LCA. The efficacy as well as the limitations of this form of treatment are evaluated in detail and recommendations are made for the future directions of gene therapy for Leber's congenital amaurosis.
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