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Flow Cytometry – Multimedia

A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor  content piece image
Poster

A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor

Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.
In-plane detection of fluorescence signals in microfludic lab-on-chip flow cytyometry content piece image
Poster

In-plane detection of fluorescence signals in microfludic lab-on-chip flow cytyometry

Several means of extracting fluorescence signals from flowing cell suspensions in a single plane are examined. Simple microfluidic flow cytometer structures incorporating lateral hydrodynamic focusing were molded in PDMS. Several geometries for embedding optical fibers and custom molded waveguides into the same structure were compared. Improvement in light yield is examined with molded cylindrical lenses and by using channels filled with high refractive index polymers as waveguides.
A simple, fast and quantitative single-step dead-cell indicator for flow cytometry content piece image
Poster

A simple, fast and quantitative single-step dead-cell indicator for flow cytometry

We have evaluated a series of new compounds for dead cell stain and identified a new product, SYTOX® AADvancedTM dead cell stain, which demonstrates improved properties over 7-AAD. These properties make the SYTOX® AADvancedTM dead cell stain a simple, fast and quantitative single-step no-wash dead-cell indicator as well as ideal for use in multicolor application requiring DNA content.
Glycoprotein Labeling and Detection: Novel Click Chemistry-based Applications for gel Electrophoresis, Flow Cytometry and Fluorescence Microscopy content piece image
Poster

Glycoprotein Labeling and Detection: Novel Click Chemistry-based Applications for gel Electrophoresis, Flow Cytometry and Fluorescence Microscopy

We demonstrate highly-selective and sensitive labeling methods for the detection of specific glycoprotein subclasses, including cell surface N- and O-linked glycoproteins and intracellular O-GlcNAc modified proteins, utilizing the copper-catalyzed cycloaddition reaction between azides and alkynes, or click chemistry.
High Throughput Cell Cycle Analysis using Microplate Cytometry content piece image
Poster

High Throughput Cell Cycle Analysis using Microplate Cytometry

To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.
Preclinical Data with siRNA Targeting WNT Pathway for Breast Cancer Treatment content piece image
Poster

Preclinical Data with siRNA Targeting WNT Pathway for Breast Cancer Treatment

The aim of present study was focused on the effect of silencing target gene in triggering of apoptosis in breast cancer MCF-7 cells. Flow cytometry, light-, confocal microscopy and viability/cytotoxicity tests were used for evaluation of the protein level, percentage of apoptotic cells and morphological features of cell death.
LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines content piece image
Poster

LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines

The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.
Applications of Laser Scanning Microplate Cytometry in High Content Screening content piece image
Poster

Applications of Laser Scanning Microplate Cytometry in High Content Screening

Traditional methods for High Content Analysis (HCA) use technologies such as flow cytometry and microscope-based imaging systems. Laser-scanning microplate cytometry has many advantages over these, and is more amenable for use in High Content Screening (HCS).
Cell Cycle Analysis Using Microplate Cytometry: A Comparison of Laser and Dye Combinations content piece image
Poster

Cell Cycle Analysis Using Microplate Cytometry: A Comparison of Laser and Dye Combinations

For improved screening capability, we have developed a cell cycle analysis method using an Acumen Explorer fluorescence microplate cytometer, capable of reading an entire 384 well microplate in about 10 minutes. The method can perform such analyses on fixed cells in situ, markedly simplifying sample preparation.
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