We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement

High-Throughput Screening – Multimedia

PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays content piece image
Poster

PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays

The multimode microplate reader PHERAstar from BMG combines the rapid plate reading necessary for HTS with enhanced performance and sensitivity needed to read small liquid volumes along with the flexibility to tackle the most demanding assays. The performance and features of the PHERAstar are presented in this poster with commercially available HTS kits such as HTRF®, AlphaScreen™, and PolarScreen™.
A Sensitive Fluorimetric Assay for Detection of ß-Secretase Activity Using a Novel FRET Peptide Substrate content piece image
Poster

A Sensitive Fluorimetric Assay for Detection of ß-Secretase Activity Using a Novel FRET Peptide Substrate

In order to facilitate high throughput screening of AD drug candidates, we have developed a new SensoLyte™ 520 b-secretase assay kit using a fluorescence resonance energy transfer (FRET) peptide, HiLyte Fluor™ 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH. The longer excitation and emission wavelengths of HiLyte Fluor™488 minimize the interference from autofluorescence and absorbance of test compounds.
High Throughput Screening with Infectious Agents: Equipment Choices and Strategies content piece image
Poster

High Throughput Screening with Infectious Agents: Equipment Choices and Strategies

At Southern Research Institute we routinely conduct HTS campaigns using infectious agents at the BSL-2 (Biosafety Level) and BSL-3 level. Here we discuss how we have successfully screened over one million compounds in anti-viral (Severe Acute Respiratory Syndrome, Influenza, Highly Pathogenic Avian Influenza) and anti-microbial (Staphylococcus aureus, Escherichia coli, Mycobacterium tuberculosis) screens in BSL-2 and BSL-3 containment.
Promega’s Multiplexed Cell Viability and Apoptosis Assays Performed on the PHERAstar content piece image
Poster

Promega’s Multiplexed Cell Viability and Apoptosis Assays Performed on the PHERAstar

Today’s high-throughput screening facilities face increasing demands to generate more information from their existing compound libraries. In this poster, we demonstrate the combination of several Promega cell-based assays (CellTiter-Blue®, Apo-One® and Caspase-Glo® 3/7) multiplexed in both low-volume 384 and 1536-well plate formats. The BMG LABTECH PHERAstar microplate reader is used to record both luminescence and fluorescence, depending on the multiplex combination.
CyTRAK™ Probes: Novel Nuclear and Cytoplasm Discriminators Compatible with GFP-Based HCS and HTS Assays content piece image
Poster

CyTRAK™ Probes: Novel Nuclear and Cytoplasm Discriminators Compatible with GFP-Based HCS and HTS Assays

Image-based high-content screening assays, demand solutions for image segmentation and cellular compartment encoding to track critical events – for example those presented by GFPreporters within cell cycle tracking and GPCR translocation assays. We have designed nuclear and cytoplasm discriminator CyTRAK™ probes - spectrally compatible with all variants of GFPreporters offering new solutions in cytometry.
An Ultra-Sensitive Fluorimetric Assay for the Detection of Renin Activity content piece image
Poster

An Ultra-Sensitive Fluorimetric Assay for the Detection of Renin Activity

To facilitate high throughput screening of renin inhibitors, we have developed new renin assay kits utilizing FRET peptide substrates. The SensoLyte™ 520 Renin Assay Kit contains FRET peptide labeled with the quencher QXL™520 and the fluorophore 5-FAM. These newly developed assays were validated with several known renin inhibitors. Assay with QXL™ 520/5-FAM substrate was approximately 40 fold more sensitive than an existing assay which uses EDANS/DABCYL FRET substrate.
Use of Nanolitre Pipetting in Hit-to-Lead Optimisation content piece image
Poster

Use of Nanolitre Pipetting in Hit-to-Lead Optimisation

Assay-ready plates are becoming key to maximizing throughput for many HTS departments. These plates contain test compounds prepared from library stocks ahead of time at the correct concentrations. DMSO is routinely used for compound dissolution, but its presence at concentrations above 1% can markedly affect assay performance. Thus, accurate nanolitre quantities of DMSO-based stock solution are required to keep concentrations low when preparing assay-ready plates.
An Ultra-High Throughput Approach to High Content Screening in 1536-Well Format content piece image
Poster

An Ultra-High Throughput Approach to High Content Screening in 1536-Well Format

The Acumen Explorer combines the object-recognition capabilities of image-based systems with read speeds similar to that of bulk readers. Here, we demonstrate the powerful integration of an Acumen Explorer with the Kalypsys® Integrated Screening System, with capability to screen > 300,000 wells per day of high content data.
Comparative Virtual and Experimental High-Throughput Screening for Glycogen Synthase Kinase-3b Inhibitors content piece image
Poster

Comparative Virtual and Experimental High-Throughput Screening for Glycogen Synthase Kinase-3b Inhibitors


Glycogen synthase kinase-3b (GSK-3b ) is a serine/threonine kinase that has recently emerged as a key target for neurodegenerative diseases and diabetes. As an initial step of our lead discovery program we developed a virtual screen to discriminate known GSK-3b inhibitors and inactive compounds using FlexX, FlexX-Pharm and FlexE.
NanoQuot™, A Cost-Effective, Flexible Nanoliter Dispenser for High Throughput Screening content piece image
Poster

NanoQuot™, A Cost-Effective, Flexible Nanoliter Dispenser for High Throughput Screening

BioTek has developed the NanoQuot™, a dispenser capable of running stand-alone or computer controlled as part of a robotics system. Positive gas pressure system, in conjunction with eight precision valves, provides accurate and precise dispensing of volumes ranging from 100 nanoliters to 40 microliters in 96-, 384-, and 1536-well plates.
Advertisement