We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement

High-Throughput Screening – Multimedia

Screening and Profiling for Kinases: Development of a Unique and Versatile Platform content piece image
Poster

Screening and Profiling for Kinases: Development of a Unique and Versatile Platform

To build a unique kinase platform providing reliable and quantitative data, we have designed a flexible system with well-defined yet different assay conditions for each kinase. This platform minimizes interferences, including those from test compounds, and displays a homogeneous and generic assay format.
High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry content piece image
Poster

High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry

Here, we model the integration of the Protedyne BioCube System and the Acumen Explorer for practical high-throughput, high content screening of protein kinases.
Miniaturization of Protein Kinase Assays in the MultiScreen®HTS 384-Well P81 Phosphocellulose Filter Plate content piece image
Poster

Miniaturization of Protein Kinase Assays in the MultiScreen®HTS 384-Well P81 Phosphocellulose Filter Plate

In this study, using Protein Kinase A (PKA) as are presentative assay system, peptide phosphorylation reactions carried out in 384 PH filter plates showed dependence on enzyme and substrate concentrations, and on time. Kinase inhibition studies demonstrated IC50 and Z’ values equivalent to those obtained in 96-well plates. The higher throughput 384 well format offers a miniaturized version of the 96 well assay with equivalent sensitivity and reproducibility.
High Density Receptor Ligand Binding Assays in the MultiScreen®HTS 384-well Glass Fiber Filter Plate content piece image
Poster

High Density Receptor Ligand Binding Assays in the MultiScreen®HTS 384-well Glass Fiber Filter Plate

Using the Muscarinic M1 G-protein Coupled Receptor as a model system, we achieved accurate and reproducible determinations of binding affinity (Kd) and IC50 results for known ligands on the 384-well plate device. As a result of being able to incubate the reaction mixture in the filter plate and configure the assay using half the reaction volume, significant reductions in reagent costs and radioactive waste were achieved.
Advertisement