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High-Throughput Screening – Multimedia

Novel Antimalarial Drug Candidates Generated In Silico by Analysis of Public HTS Data content piece image
Poster

Novel Antimalarial Drug Candidates Generated In Silico by Analysis of Public HTS Data

This research aims to provide proof-in-principle that in silico tools could be applied to public data so as to efficiently identify active chemistry with good ADMET properties.
App Note / Case Study

Automation of a Generic Fluorescent Methyltransferase Activity Assay

The primary function of this application note is to demonstrate automated methods for a HTS-ready universal methyltransferase activity assay.
Effect of Liquid Handling Quality Control on Biological Assay Performance content piece image
Poster

Effect of Liquid Handling Quality Control on Biological Assay Performance

High quality assays are required in order to take forward potential drugs, post screening. Therefore the liquid handling must be accurate and precise, as demonstrated in this experiment.
Automation of a Generic Fluorescence Methyltransferase Activity Assay content piece image
Poster

Automation of a Generic Fluorescence Methyltransferase Activity Assay

Epigenetic processes are attracting considerable attention in drug discovery as their fundamental roles in controlling normal cell development and contributions to disease states become more clearly defined. This work combines a fluorescence-based assay with liquid handling and dispensing instrumentation and a multi-mode reader which can be used to monitor the biological activity of the histone methyltransferase (HMT) G9a, a model system.
App Note / Case Study

Characterization of Emission Properties of Aequorin Mutants with Rapid, High Throughput Screening Assay

This application note explains how to perform a rapid, high throughput screening assay of mutated photoprotein aequorin in order to find clones with more intense, slower kinetic, narrow spectral or color-shifted bioluminescent emission. Several examples of bioluminescent emission kinetics and spectra of mutated aequorins are included.
App Note / Case Study

Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader

Two cell-based signaling assays from Invitrogen, Fluo-4 Direct™ Calcium Assay and Tango™-bla U2OS GPCR Assay, were performed on the PHERAstar FS HTS instrument. The PHERAstar FS has several unique features that enhance the performance of these assays including direct optic bottom reading, high resolution cell layer scanning, injection at the point of measurement and dual emission detection.
App Note / Case Study

Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader

Two cell-based signaling assays from Invitrogen, Fluo-4 Direct™ Calcium Assay and Tango™-bla U2OS GPCR Assay, were performed on the PHERAstar FS HTS instrument. The PHERAstar FS has several unique features that enhance the performance of these assays including direct optic bottom reading, high resolution cell layer scanning, injection at the point of measurement and dual emission detection.
Fragment Screening Using Capillary Electrophoresis content piece image
Poster

Fragment Screening Using Capillary Electrophoresis

Capillary electrophoresis, which was pioneered for HTS in the 1990s, has recently been adapted to enable the screening of fragments. The technique is explained and the screening of fragments against a chaperone protein Hsp90 is presented as an example.
Some thiazole derivatives as potent antiproliferative agents with CDK2/Cycin E/A inhibitory activities content piece image
Poster

Some thiazole derivatives as potent antiproliferative agents with CDK2/Cycin E/A inhibitory activities

We report synthesis and biological evaluation of novel thiazole derivatives. High throughput screening of various compounds for their anti-proliferative activity led to the identification of thiazolo-thiones as potential candidate for Lung cancer (NCl-H23, NClH510A, NCI-H522) and breast cancer (MDA-MB-453/231/468, MCF-7). The most potent compounds were also screened for their inhibitory activity against CDK2/ Cyclin E/A which are considered as promising targets for lung and breast cancer.
Real-Time Monitoring of ATP Depletion Suitable for HTS content piece image
Poster

Real-Time Monitoring of ATP Depletion Suitable for HTS

ATP depletion assays of kinases, ATPases and aminoacyl tRNA synthetases can be performed in the presence of firefly luciferase/luciferin, by monitoring the light emission continuously. Such assays are preferably set up to result in a first order reaction rate with respect to ATP consumption.
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