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Light Microscopy – Multimedia

A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles. content piece image
Poster

A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles.

We developed a microfluidic device for encapsulating gold nanoparticles in Micro-Alginate Beads (MABs). The size and the gap of the monodisperse alginate droplets were successfully controlled by adjusting the relative "oil/sample" flow rate ratio. Droplets reacted with calcium ion at the interface between oil phase and aqueous phase so that MABs precipitated spontaneously and undergone complete gelation. Purified MABs were successfully observed by fluorescence microscopy.
Glycoprotein Labeling and Detection: Novel Click Chemistry-based Applications for gel Electrophoresis, Flow Cytometry and Fluorescence Microscopy content piece image
Poster

Glycoprotein Labeling and Detection: Novel Click Chemistry-based Applications for gel Electrophoresis, Flow Cytometry and Fluorescence Microscopy

We demonstrate highly-selective and sensitive labeling methods for the detection of specific glycoprotein subclasses, including cell surface N- and O-linked glycoproteins and intracellular O-GlcNAc modified proteins, utilizing the copper-catalyzed cycloaddition reaction between azides and alkynes, or click chemistry.
SPR Imaging of Protein Microarrays for Detection of Antibody Binding content piece image
Poster

SPR Imaging of Protein Microarrays for Detection of Antibody Binding

In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
Preclinical Data with siRNA Targeting WNT Pathway for Breast Cancer Treatment content piece image
Poster

Preclinical Data with siRNA Targeting WNT Pathway for Breast Cancer Treatment

The aim of present study was focused on the effect of silencing target gene in triggering of apoptosis in breast cancer MCF-7 cells. Flow cytometry, light-, confocal microscopy and viability/cytotoxicity tests were used for evaluation of the protein level, percentage of apoptotic cells and morphological features of cell death.
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