Light Microscopy – Multimedia

Video
Machine Learning and Image Analysis Methods in High-content Screening for Phenotypic Drug and Gene Discovery
Peter Horvath discusses image correction and machine learning methods for phenotypic discovery.

Poster
Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering
Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.

Poster
Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks
We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.

Poster
Automated Method to Determine Infectious Dose (TCID50)
Settings for each cell type and virus can be saved and re-used to assure objective results. Additional information such as cell health, cell cycle status can be determined from the same plates using Nexcelom designed applications. Time course studies can be carried out by scanning plates over several days/weeks allowing for additional information than may be generated by carrying out end-point assays alone.

Poster
Automated Method to Determine Infectious Dose (TCID50)
Settings for each cell type and virus can be saved and re-used to assure objective results. Additional information such as cell health, cell cycle status can be determined from the same plates using Nexcelom designed applications. Time course studies can be carried out by scanning plates over several days/weeks allowing for additional information than may be generated by carrying out end-point assays alone.

Poster
Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies
Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.

Poster
Digital microphotographic atlas of the adult zebrafish cerebellum
Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.

App Note / Case Study
Measurement of nuclear translocation in primary T cells using correlation analysis of images obtained on the ImageStream®
Molecular translocation of transcription factors from the cytoplasm to the nucleus is a pivotal event in many processes critical to cellular activation, diff erentiation and host defense. Existing methods used to measure nuclear translocation have significant limitations.

Poster
A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles.
We developed a microfluidic device for encapsulating gold nanoparticles in Micro-Alginate Beads (MABs). The size and the gap of the monodisperse alginate droplets were successfully controlled by adjusting the relative "oil/sample" flow rate ratio. Droplets reacted with calcium ion at the interface between oil phase and aqueous phase so that MABs precipitated spontaneously and undergone complete gelation. Purified MABs were successfully observed by fluorescence microscopy.

Poster
Glycoprotein Labeling and Detection: Novel Click Chemistry-based Applications for gel Electrophoresis, Flow Cytometry and Fluorescence Microscopy
We demonstrate highly-selective and sensitive labeling methods for the detection of specific glycoprotein subclasses, including cell surface N- and O-linked glycoproteins and intracellular O-GlcNAc modified proteins, utilizing the copper-catalyzed cycloaddition reaction between azides and alkynes, or click chemistry.
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