Western Blot – News and Features

Having grown beyond early ‘proof-of-principle’ studies, top-down proteomics is now extensively used to analyze intact proteins in numerous applications. Here, we discuss how the top-down proteomics approach emerged, reasons underpinning its success, and highlight some of the most exciting current developments.

How does the gut talk to the brain? In this new case study from ProteinSimple, we find out how Melanie Maya Kaelberer, a Postdoctoral Associate at Duke University, is using Single-Cell Western platform Milo to answer the question of how the gut can rapidly communicate with cranial nerves.

This article outlines key workflow steps to help researchers perform better western blots. By executing best practices in these steps – sample preparation, gel electrophoresis, gel transfer, protein detection, and image analysis – researchers can expect good western blot data.

Presence of host cell protein (HCP) contaminants in the final product, even at very low levels, may elicit unpredictable and serious immunogenic response in humans. The accurate detection, identification and quantification of HCPs during biotherapeutic development is thus critical in ensuring patient safety, and is a regulatory requirement.

They say 'a picture is worth a thousand words.' Is this true for cell images?

A western blot is supposed to give you information on true experimental conditions or differences between samples. It is instrumental for the detection of proteins in research discovery and biopharma. In this article, we will be explaining the two main western blotting normalization methods; the older housekeeping protein method and the newer TPN method.

Here we take a look at the use of microfluidic devices in the isolation and analysis of circulating tumor cells.

The current OEM agreement is expanded to cover two additional product lines, with an expected annual revenue contribution now more than EUR 1M.

2016 has seen the release of some truly innovative products. To help you digest these developments, The Scientist have listed their top picks for the year.

There are three main kinds of cytoskeletal filaments: microfilaments, microtubules, and intermediate filaments in Eukaryotic cells. Intermediate filaments help to provide structure to cells, and are involved in cell movement.