Episode 3: Light-Seq: Light Directed Spatial Indexing and Sequencing
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Light-Seq is a novel approach for multiplexed spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues, followed by ex situ sequencing. This method combines rapid, spatially targeted photocrosslinking of DNA barcodes in situ with a streamlined one-step DNA stitching reaction, enabling the creation of pooled, spatially indexed sequencing libraries.
With Light-Seq, researchers can define regions of interest (ROIs) — from large tissue structures down to single cells — to capture transcriptomic information with precision. The platform has been successfully applied to cultured cells, distinct layers of the mouse retina, and rare neuronal subtypes such as dopaminergic amacrine cells. This flexibility makes Light-Seq an enabling technology for a wide variety of applications in spatial transcriptomics.
Attend this webinar to:
- Discover how Light-Seq enables high-resolution, light-directed spatial indexing
- Explore applications from cell culture to rare neuronal subtypes
- Learn how user-defined ROIs unlock transcriptomic analysis from tissue regions to single cells
