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Application Notes

 
The Diffusion Interaction Parameter (kD) as an Indicator of Colloidal and Thermal Stability
Application Note

WITec

This experiment demonstrates that thermal and colloidal stability of proteins, two indicators of propensity to aggregate, as well as actual aggregation states, are all determined simultaneously during the screening process with dynamic light scattering (DLS) tools in order to rank the effectiveness of candidates and formulation conditions.

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Accelerating the Development of Optimized Therapeutic Protein Formulations using Differential Scanning Calorimetry
Application Note

Malvern Panalytical

DSC data is useful in predicting the stability of a protein in solution. The Tm indicates thermostability, and Tm determination in different formulations is an approximate measure of the susceptibility to aggregation and other irreversible changes at lower temperatures.

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Differentiation and characterization of subvisible particulates in therapeutic protein products
Application Note

Malvern Panalytical

The formation of protein aggregates is a particular concern for parenteral administration biopharmaceuticals due to the potential for increased immunogenicity.

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An Introduction to Proteins in 30 minutes
Application Note

Malvern Panalytical

In the biotechnology industry, the majority of research is directed towards characterizing the structure and function of proteins. New methods are continually being developed to accomplish this.

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Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Application Note

Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.

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Tag-lite® two-cell assay: a valuable tool for protein drug discovery
Application Note

Tecan Group Ltd.

Antibodies are central mediators of the immune system, IgG being the most dominant. Binding of IgG/antigen complexes to membrane-bound Fc? receptors triggers a cellular immune response. Alterations in the Fc portion of the antibody by introducing mutations can lead to complete abolition of Fc? receptor binding. This describes the development of a cellbased TR-FRET assay to determine the simultaneous binding of an antibody to its antigen and huFc?RIIIa present on different cells.

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3D Cell Culture: mimicking real tissue
Application Note

Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory.

Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.

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Protein Purification Workflow Development Using Bio-Rad's NCO' Chromatography System
Application Note

Bio-Rad Laboratories

High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. This is, however, not always a viable option. This application note discusses protein purification workflow development for untagged proteins and introduce a new indicator of method performance, the purity quotient difference.

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Direct Visualisation, Sizing and Counting of Aggregation in Proteins
Application Note

NanoSight Limited

Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.

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Direct Visualisation, Sizing & Counting of Viruses and Phages in Liquid Suspension
Application Note

NanoSight Limited

A new laser-based nanoparticle tracking analysis system is now available which allows nanoscale particles such as viruses and virus aggregates to be directly and individually visualised in liquids in real-time, from which high-resolution particle size distribution profiles can be obtained.

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