A Highly Robust and Sensitive Workflow for Quantifying RTP004 in Rat Serum

A Highly Robust and Sensitive SPE-LC-HRMS Workflow for Quantifying KR Rich Peptide RTP004 in Rat Serum

RTP004 is a proprietary peptide used by Revance Therapeutics as a novel excipient in DaxibotulinumtoxinA for injection. Due to its high molecular weight, high isoelectric point and strong non-specific binding property, the sample preparation and LC-MS method development for this peptide is very challenging.

Download this app note to discover how a SPE-LC-HRMS workflow: 

• Enabled accurate quantitation of RTP004 in rat serum
• Provides high sensitivity, resolution, mass accuracy and robustness of large peptides
• Can easily be applied to other large peptides with low fragmentation efficiency