Optimize Antibody Internalization Studies
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Antibody internalization underpins applications from ADC delivery to receptor modulation.
This application note presents a live-cell, no-wash assay using a pH-sensitive Fab-based labeling dye to quantify internalization events as antibodies traffic to acidic compartments.
The workflow pairs one-step labeling with automated kinetic imaging on a live-cell imaging platform, enabling side-by-side comparisons across many antibody candidates in miniaturized plate formats.
Download this app note to discover:
- A simplified, high-throughput workflow for real-time quantification of antibody internalization (no wash steps)
- Scalable 96-/384-well workflows applicable across multiple cell types and targets
- Example data, including anti-CD71 uptake in HT-1080 cells and lysosomal localization of internalized signal
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Further information on the processing of personal data can be found in the Sartorius Privacy Notice.
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