Characterization and Relative Quantification of mRNA 5’-Capping

Products based on mRNA have broad potential for use as therapeutics, and because mRNA leverages the translational machinery of the host cells, there is a significantly reduced risk of unwanted post-translational modifications. Nonetheless, mRNA-based products have other critical quality attributes that directly impact both the efficacy and safety of the product, one example of this being the 5’-capping of mRNA to maintain translational efficiency.

Download this app note to discover a method that allows:

• Fast and reliable identification of the 5’-end structure of IVT mRNA species
• Straightforward and fully customizable quantification of mRNA capping efficiency
• Provides high confidence in identification of main and low-abundance species