Comprehensive Characterization of O-Linked Glycosylation in Etanercept by Electron Activated Dissociation
Glycosylation is a common post-translational modification (PTM) that plays a critical role in antibody effector functions. Comprehensive characterization of N- and O-linked glycosylation in protein therapeutics is essential for ensuring drug safety and efficacy.
Accurate determination of glycosylation sites using traditional collision-based MS/MS approaches is extremely challenging.
Download this app note to learn how electron activated dissociation can:
- Preserve the glycan structures in glycopeptide fragments
- Provide confident identification and accurate localization of O-glycosylation
- Be leveraged to elucidate complex glycosylation profiles in protein therapeutics