The New Gold Standard for Gene Therapy Protein Expression Potency Measurements
Simple Western™: Analytical-Grade Protein Expression Quantification in a Multi-Attribute Platform Approach
A major challenge preventing transformative gene therapies from reaching patients is the lack of fit-for purpose potency assays, which are some of the most important assays in an analytical package but are often the most complex and high-risk.
Download this app note to discover a method with parallel line analysis for AAV-mediated gene delivery in cultured human cells. Specifically, this method demonstrates:
- Relative protein expression potency between AAV serotypes with high specificity and <5% CVs
- Absolute protein expression measurements in AAV transduced cells using a recombinant standard curve
- Detection of residual intracellular capsid proteins in AAV transduced cells as an infectivity surrogate