Quantitative Cell Profiling of Immune Cell Types From Whole Blood Samples
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Exploring cellular heterogeneity and immune composition from whole blood samples can provide prognostic insight and be used to predict therapeutic response.
However, traditional cell profiling methods can only interrogate a limited number of markers per assay. More recently, single cell RNA-sequencing has been adopted, but is limited by sample preparation artifacts and high costs.
This application note explores an optimized RNA-sequencing workflow that reliably determines cell subset abundance and cell-type-specific gene expression profiles from bulk tissue or blood expression data.
Download this application note to learn more about:
- Robust, accurate and reproducible enumeration of immune cell subsets from whole blood samples
- Target enrichment for targeted RNA sequencing
- iSort software to quantify immune cells from bulk transcriptome data
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