Unveiling Hidden Insights: Enhanced Analytics with New Heights of Sensitivity
Credit: Sartorius
In this document, we have presented data demonstrating accurate detection of analyte concentrations as low as 3.9 ng/mL (26 pM) on the Octet® R8e system. Additionally, the improved signal-to-noise ratios allow for more flexibility in assay orientation, where avidity effects are a concern, resulting in more physiologically relevant insights. Compatibility with 384-well microplates enables high-throughput assays with minimal sample volumes as low as 40 μL, which enhances efficiency and reduces reagent costs for labs seeking operational efficiency.
While SPR has historically been considered the gold standard of label-free assays for biomolecular interaction analysis, the next-level sensitivity of the Octet® R8e system unlocks the potential of BLI as an alternative for applications with small molecules or low-analyte samples. Moreover, unlike SPR platforms, the Octet® R8e eliminates the need for microfluidics, makes loading times and cleaning redundant, reduces the risk of clogging and contamination, and simplifies maintenance.
The innovative evaporation control feature for the 96-well microplate format maintains sample concentration stability for up to 16 hours, ensuring reliable data collection for long-term kinetic measurements required to observe slow binding interactions or dissociations, enhancing data precision and reliability.
The innovative evaporation control feature for the 96-well microplate format maintains sample concentration stability for up to 16 hours, ensuring reliable data collection for long term kinetic measurements required to observe slow binding interactions or dissociations, improving data precision and reliability. The classic values of Octet®, such as assay flexibility, user-friendly design and 21CFR Part 11 compliance for GxP laboratory settings, are now complemented by enhanced sensitivity and faster data acquisition rate capabilities. These advancements empower researchers to uncover insights previously inaccessible using BLI, accelerating the path to therapeutic breakthroughs.
While SPR has historically been considered the gold standard of label-free assays for biomolecular interaction analysis, the next-level sensitivity of the Octet® R8e system unlocks the potential of BLI as an alternative for applications with small molecules or low-analyte samples. Moreover, unlike SPR platforms, the Octet® R8e eliminates the need for microfluidics, makes loading times and cleaning redundant, reduces the risk of clogging and contamination, and simplifies maintenance.
The innovative evaporation control feature for the 96-well microplate format maintains sample concentration stability for up to 16 hours, ensuring reliable data collection for long-term kinetic measurements required to observe slow binding interactions or dissociations, enhancing data precision and reliability.
The innovative evaporation control feature for the 96-well microplate format maintains sample concentration stability for up to 16 hours, ensuring reliable data collection for long term kinetic measurements required to observe slow binding interactions or dissociations, improving data precision and reliability. The classic values of Octet®, such as assay flexibility, user-friendly design and 21CFR Part 11 compliance for GxP laboratory settings, are now complemented by enhanced sensitivity and faster data acquisition rate capabilities. These advancements empower researchers to uncover insights previously inaccessible using BLI, accelerating the path to therapeutic breakthroughs.
