Best Practices for In Vitro Transcribed mRNA Analysis
The ability to synthesize mRNA using in vitro transcription (IVT) has advanced the development of novel therapeutics and diagnostics tools. However, the efficacy of IVT final products can be easily influenced by temperature, incubation time and secondary structure.
For IVT to succeed robust quality control processes are required; they must accurately and reliably measure the quality and size of transcribed mRNA and gauge the purity of the final product.
This guide highlights crucial sample handling techniques and standards for IVT analysis.
Download this guide to learn about:
- The latest sample handling techniques for accurate and reliable IVT mRNA analysis
- The role of chemical and temperature denaturation in sample preparation
- The importance of sample quantification before fragment analysis