Western blotting is a cornerstone of many laboratories, but can have a complex, lengthy (even multi-day) protocol, requiring staining, destaining and probing for housekeeping proteins.
Traditional western blotting instruments are typically large and shared with other groups. A smaller benchtop reader can offer improved productivity and still capture large-format gels without compromising acquisition, quantitation or accuracy.
Discover the Bio-Rad ChemiDoc™ Go, a versatile, compact platform compatible with a range of detection modes and multiple light sources for precise illumination.
Download this handbook to explore:
- A complete, stain-free western blotting solution
- Simple, secure cloud storage
- The advantages of fluorescent and multiplex blotting
Capture gel and western blot images from your benchtop
with the same sensitivity as with larger, shared instruments.
With advanced complementary metal oxide semiconductor (CMOS) digital imaging and high-intensity light sources,
the ChemiDoc Go Imaging System offers no compromises in sensitivity or dynamic range. Choose traditional
chemiluminescence or colorimetric detection or go further using multiplex analysis with cost-effective StarBright Blue
Fluorescent Secondary Antibodies. Normalize to total protein with Stain-Free gels in your westem blot workflow and
eliminate the need to detect housekeeping proteins.
The ChemIDoc Go Imaging System features high-Intensity, all-LED Illumination sources.
Transillumination
UVLEDs: higher Inteneity at precise wavelengths
Blue LEDs: higher-Intensity, precise-wavelength
Ilumination for SterBright Blue 520 end 700
Antlbodes, safe nuclelc acid stelns, and nuclelc acld
band excislon
Amber LEDs: higher-Intensity, precise-wavelength
Ilumination ylelds greater signal Intensity with
Coomassle Blue and other vislble steins
Complete Stain-Free
Western Blotting Solution
Using Stain-Free technology in your western blot workflow
can help you achieve accurate results in less time.
With Stain-Free imaging, you can assess the quality of sample separation after electrophoresis and the efficiency
of protein transfer and then normalize your data to total protein - all without staining, destaining, or probing for
housekeeping proteins. Streamline your day with a ChemiDoc Go Imaging System on your bench.
Visit bio-rad.com/StainFree to learn more about Bio-Rad's Stain-Free Westem Blotting Workflow.
Fluorescently labeled secondary antibodies and advanced digital imaging
are a powerful combination for western blot detection and quantitation.
The ChemiDoc Go Imaging System allows fluorescence detection in two colors using StarBright Blue 520 and 700
Fluorescent Secondary Antibodies. Fluorescence detection with StarBright Blue Dye is compatible with total protein
normalization using Stain-Free technology, eliminating the need for a channel to detect housekeeping proteins.
Three-Step Touch Workflow
Using Image Lab Touch Software, choose the optimized
preset for your application, select Acquire, and save the
image in your choice of multiple file formats. Save to your
institution's network, a USB drive, or your BR.io cloud
account, and even print to a dedicated printer. Analyze
your data anywhere with Image Lab Software for Mac
or PC. Maintain electronic records for U.S. FDA 21
CFR Part 11 compliance with Image Lab Software,
Security Edition for PC.
Secure Cloud Storage
The first Bio-Rad imager connected to the BR.io
cloud platform, the ChemiDoc Go System simplifies
data storage, sharing, and analysis when images
are uploaded to your secure folders in the doud.
Visit BR.io for more information on the powerful
capabilities of the BR.io cloud platform.
Advanced CMOS Sensor Technology
After years of development, CMOS sensor technology
now meets the demanding requirements of life science
imaging, delivering higher efficiency and greater pixel
density than charge-coupled device (CCD) detection.
The new high-sensitivity CMOS sensors of the ChemiDoc
Go Imaging System also require less cooling and power,
enhancing reliability.
StarBright Compatibility
The ChemiDoc Go System now supports imaging
using StarBright Blue 520 and 700 Fluorescent
Secondary Antibodies.
All-LED Illumination
New in the ChemiDoc Go System are all-LED light sources
for trans- and epi-illumination. Multiple light sources provide
precise illumination for each application, and all-LED ights
eliminate the need for bulb replacements.
Why StarBright Fluorescent
Secondary Antibodies
StarBright Blue fluorescent nanopartides provide a
considerably brighter signal than conventional dyes and
match the most sensitive chemiluminescent substrates.
These fluorescent nanoparticles are conjugated to highly
cross-absorbed goat anti-mouse (GAM) or goat anti-rabbit
(GAR) IgG and are easily adapted to your current protocol.
Eliminate the need for detection substrates and take
advantage of low background and a photostable signal
when using StarBright Blue Secondary Antibodies.
Why Multiplex Blotting
Multiplex blotting allows for the simultaneous detection
and quantitation of multiple proteins on a single blot. In
addition to saving time, the many advantages of multiplex
detection include:
. No stripping, reprobing, or cutting the membrane
. Conserving precious sample
. Generating more information from each blot
. Visualizing modified and unmodified targets with
posttranslational modifications at one time
. Quantitating proteins close in molecular weight
with accuracy