We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Highly Sensitive ELISA Technique for Bevacizumab Bioanalysis

Highly Sensitive ELISA Technique for Bevacizumab Bioanalysis

Highly Sensitive ELISA Technique for Bevacizumab Bioanalysis

Highly Sensitive ELISA Technique for Bevacizumab Bioanalysis

This is a schematic diagram of the ELISA for Bevacizumab. Credit: Bentham Science Publishers and Dr. Manal A. El-Gendy et al.
Read time:

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Highly Sensitive ELISA Technique for Bevacizumab Bioanalysis"

First Name*
Last Name*
Email Address*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

This newly published article explores the development and validation of a new enzyme-linked immunosorbent assay (ELISA) with high sensitivity and selectivity for bioanalysis of bevacizumab (BEV), a monoclonal antibody used for immunotherapy of different types of cancer. BEV was captured by specific specific antigen human vascular endothelial growth factor protein; (VEGF) immobilized onto a 96-well assay plate.

The BEV-VEGF complex formed onto the plate wells were quantified using horseradish peroxidase labeled anti-human IgG (HRP-IgG) and 3,3`,5,5`-tetramethylbenzidine (TMB) as a chromogenic substrate for peroxidase enzyme. The optimum conditions for conducting the proposed ELISA were established and its analytical performance was evaluated as per the guidelines for the validation of immunoassays for bioanalysis of therapeutic monoclonal antibody. The assay limit of detection was 1.01 ng /mL and the effective working dynamic range was 3.07-100 ng/mL.

The accuracy and precision of the assay were proved. The present assay with high throughput analysis very easy to perform in a 96-well plate and permits an operator to analyze a batch of ~ 200 samples, in triplicate. This facilitates the processing of a large number of samples in a clinical setting. ELISA eliminated the need for pretreatment of plasma samples by affinity chromatography or other sophisticated equipment. The proposed ELISA is a useful and powerful method in estimating the nano - gram level of BEV. It has been widely used in the life science researches application.

The basic principle of an ELISA is to use an enzyme to detect the binding of antigen (Ag) antibody (Ab) complex. The enzyme converts a colorless substrate (chromogen) to a colored product, indicating the presence of Ag: Ab complex binding formation avoiding hazard of radiation which comes from the use of radioactive isotopes. The proposed ELISA for BEV is expected to contribute in studying its PK, PD, TDM, and assessing the expected bioavalilability of biosimillars or biobetters.

This article has been republished from materials provided by Bentham Science Publishers. Note: material may have been edited for length and content. For further information, please contact the cited source.