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Monoclonal Antibodies Crucial to Fighting Emerging Infectious Diseases

News   Mar 09, 2018 | Original story from the National Institute of Allergy and Infectious Diseases (NIAID)

 
Monoclonal Antibodies Crucial to Fighting Emerging Infectious Diseases

Panel A shows early techniques to collect polyclonal serum from individuals recovering from disease after infectious virus is cleared and antibody response has been generated. Panel B shows monoclonal antibody isolation from mice, using antigen introduction into mice, collection of B cells from mouse spleens, and production of fully mouse, antigen-specific monoclonal antibodies. Panel C shows a technique for isolation of monoclonal antibodies from humans, using antigen introduction (through natural infection or immunization), collection of immune cells from peripheral blood or lymphoid tissue, selection of B cells expressing antibodies of interest (e.g., using flow cytometry), and production of fully human, antigen-specific monoclonal antibodies. Credit: DOI: 10.1056/NEJMp1802256

 
 
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