Mouse Brain Study May Reverse Century-Old Understanding of Axon Shape

Biology textbooks may need a revision, say Johns Hopkins Medicine scientists, who present new evidence that an armlike structure of mammalian brain cells may be a different shape than scientists have assumed for more than a century.

Their study on mouse brain cells shows that the cells’ axons — the armlike structures that reach out and exchange information with other brain cells — are not the cylindrical tubes often pictured in books and on websites but more like pearls on a string.

A report on the findings is published online Dec. 2 in Nature Neuroscience.

“Understanding the structure of axons is important for understanding brain cell signaling,” says Shigeki Watanabe, Ph.D., associate professor of cell biology and neuroscience at the Johns Hopkins University School of Medicine. “Axons are the cables that connect our brain tissue, enabling learning, memory and other functions.”

Scientists have known that pearl-like structures in axons, referred to as axon beading, can develop in dying brain cells and in people with Parkinson’s and other neurodegenerative diseases due to the loss of membrane and skeletal integrity in neurons.

Under normal conditions, axons are thought to be shaped like tubes with a mostly constant diameter and occasional bubble-like structures (synaptic varicosities that hold globs of neurotransmitters, which enable signaling to other brain cells).

Watanabe had initially seen repeated axon pearling in the nervous system of worms and grew more curious about the structures after a discussion with Swiss scientist Graham Knott, Ph.D. A research team from Harvard University had published a study in 2012 that identified repeated “skeletal” components in axons, so the pair of researchers discussed experiments to get rid of the axon skeleton to see if the pearl structures disappear, says Watanabe.

Johns Hopkins graduate student and study first author Jacqueline Griswold tested the idea but found no effect on axon pearling.

Then, Watanabe and Griswold worked with theoretical biophysics colleague Padmini Rangamani, Ph.D., professor of pharmacology at University of California San Diego School of Medicine, to look more closely at axons’ physical properties.

To be able to see axons on brain cells (neurons), which are 100 times smaller than the width of a human hair, the scientists used high pressure freezing electron microscopy. Like standard electron microscopy, which shoots beams of electrons at a cell to outline its structure, Watanabe and his team froze mouse neurons to preserve the structures’ shape.

“To see nanoscale structures with standard electron microscopy, we fix and dehydrate the tissues, but freezing them retains their shape — similar to freezing a grape rather than dehydrating it into a raisin,” says Watanabe.

The researchers studied three types of mouse neurons: ones grown in the lab, those taken from adult mice and those taken from mouse embryos. The neurons were nonmyelinated (they were without the myelin insulating cover that surrounds the axon).

The researchers found the bubbly, pear shape of axons among all of the tens of thousands of images taken of the tissue samples.

The scientists named the pearl-like structures in which the axon swells “non-synaptic varicosities.”

“These findings challenge a century of understanding about axon structure,” says Watanabe.

The scientists also used mathematical modeling to see if the axon membrane influenced the shape or presence of the pearl on a string structure. They found that simple mechanical models could be used to explain these structures very effectively.

Furthermore, experiments with the mathematical model and mouse brain samples showed that increasing the concentration of sugars in the solution around the axon or decreasing tension in the axonal membranes reduced the pearl structures’ size.

In another experiment, the scientists removed cholesterol from the neuron’s membrane to make it less stiff and more fluid-like. Under this condition, they found less pearling in both mathematical models and mouse neurons, along with reduced ability of the axon to transmit electrical signals.

“A wider space in the axons allows ions [chemical particles] to pass through more quickly and avoid traffic jams,” says Watanabe.

The scientists also applied high frequency electrical stimulation to the mouse neurons, which made pearled structures along axons swell, on average, 8% longer and 17% wider for at least 30 min after stimulation and increased the speed of electrical signals. However, when cholesterol was removed from the membrane, the axon’s pearls lost their swollen state and had no change in the speed of electrical signals.

Reference: Griswold JM, Bonilla-Quintana M, Pepper R, et al. Membrane mechanics dictate axonal pearls-on-a-string morphology and function. Nat Neurosci. 2024. doi: 10.1038/s41593-024-01813-1

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