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Maximize mRNA Yield Using In Vitro Transcription

Maximize mRNA Yield Using <i>In Vitro</i> Transcription  content piece image

The growing use of RNA-based vaccines and therapies has focused much attention and effort on developing conditions for maximizing the yield and efficiency of the in vitro transcription (IVT) reactions used to produce mRNA. IVT procedures involve the biosynthesis of RNA from a linear DNA template containing an appropriate transcription promoter. But how do different buffer conditions and nucleotide concentrations impact the IVT reaction?

Download this poster to explore how the highest mRNA yield and quality for an IVT reaction were achieved by:

  • Determining the most significant factors impacting IVT
  • Determining IVT buffer conditions to optimize mRNA yield
  • Confirming buffer and nucleotide concentration effects on mRNA yield and purity