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Protein analysis, protein concentration, protein sizing, antibody analysis, antibody qc, protein quality control, ProteoAnalyzer, enzyme, cell lysate analysis, protein expression, antibodies, protein qc
Credit: Agilent


New Agilent ProteoAnalyzer System: Automate Your Protein Analysis

New Agilent ProteoAnalyzer System: Automate Your Protein Analysis
Credit: Agilent

The Agilent ProteoAnalyzer system is an automated quality control (QC) instrument designed to provide accurate and precise quality measurements of protein samples. The system analyzes proteins based on migration time and fluorescence intensity using parallel capillary electrophoresis with sodium dodecyl sulfate (CE-SDS). Rapid and consistent sizing and quantification results can be achieved for both reduced and nonreduced protein samples.

The ProteoAnalyzer system analyzes up to 12 samples simultaneously in one run in approximately 30 minutes. Accurate sizing can be achieved for both small and large samples, ranging in size from 10 to 240 kDa. Samples can be detected over a wide concentration range, from 2 to 2,000 ng/µL. The discrete individual capillaries are rejuvenated between every separation allowing for the analysis of diverse samples including monoclonal antibodies, biosimilars, protein purification fractions, and crude cell lysates.

The ProteoAnalyzer is a robust system, well-suited for protein applications in biotherapeutics and synthetic biology workflows, enabling scientists to efficiently perform various protein quality and quantity assessments using the Agilent Protein Broad Range P240 kit. 

Key advantages: 

Analyze challenging sample types—using discrete capillaries for separations, purified antibodies, secreted proteins, membrane proteins, and crude lysates can be consistently and simultaneously assessed

Increase lab efficiency through automation—automated instrument preparation, separation, and streamlined protein labeling procedures allow you to analyze more samples per day

Get consistent results between runs—the ProteoAnalyzer system automatically cleans, reconditions, and refills capillaries between every sample, leading to reliable results

Take advantage of digital data—automated data processing and exportable data files reduce analysis time and simplify data archiving and recovery

The ProteoAnalyzer system adds efficiency, versatility, and reliability, particularly in your protein QC workflows.

For Research Use only. Not for use in diagnostic procedures. PR7001-2107

Product Specifications
Sizing range: 10 to 240 kDa and 10 to 200 kDa, depending on method used
Typical Sizing Accuracy: < 15% for BSA, CAII and < 10% for BSA, CAII, depending on method used
Typical resolution: <10% molecular weight resolution between 15 to 150 kDa (based on ladder)
Sizing Precision: <5% - <10% for standard proteins, depending on method used
Quantitative Range: 2 ng/µL to 2,000 ng/µL for BSA in PBS
Sensitivity (S/N >3): 1 ng/µLfor BSA, CAII in PBS
Quantification Reproducibility: <15 %CV for 20-2,000 ng/µL and <25 %CV for 2-20 ng/µL BSA
Total Run Time: 30 minutes
Samples Per Run: 11 samples + ladder in well 12
Sample Volume Required 1 µL
About Agilent Technologies
Agilent Technologies is a global leader in life sciences, diagnostics and applied markets, recognized for uncompromising integrity in all they do. Their mission is to advance quality of life by focusing their expertise in six key markets. With a team of approximately 18,000 employees spread across the world, their global footprint includes sales offices, logistics centers and business facilities in three major geographies. Their worldwide capabilities enable them to deliver high-quality solutions to their valued customers in 110 countries.
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