Thermo Scientific™ MarqMetrix™ Process Raman Analyzer: Real-Time PAT for Upstream and Downstream

The Thermo Scientific™ MarqMetrix™ All-In-One Process Raman Analyzer is a compact, powerful solution for continuous Process Analytical Technology (PAT) monitoring across both upstream and downstream biopharmaceutical processes. Built with industry-leading optics, adaptable probe technology, and seamless integration with existing bioprocess systems, it delivers real-time molecular insights that accelerate decision-making, enhance product quality, and streamline operations.

Upstream Bioprocess Applications

Upstream biopharma manufacturing focuses on cell culture and fermentation, where process monitoring is essential to optimize cell growth and productivity. The MarqMetrix All-In-One Process Raman Analyzer acts as the “eye of the bioreactor,” providing in-line, non-destructive analysis of key metabolites within minutes—without the need for sampling. In a single scan, the analyzer measures glucose, lactate, glutamine, glutamate, viable cell density (VCD), titer, and more, enabling precise feedback control of culture conditions.
Seamless integration with Thermo Scientific™ DynaDrive™ bioreactor and TruBio™ software allows biopharma teams to achieve fully automated bioreactor operation. For example, glucose and VCD feedback loops have been verified across multiple perfusion runs, reducing manual interventions and ensuring consistent, high-yield performance. Shared application notes demonstrate the combined value of MarqMetrix All-In-One Process Raman Analyzer with DynaDrive bioreactor and TruBio software, reinforcing its role in modern PAT strategies.

Downstream Bioprocess Applications

In downstream processing, where purification and recovery define final product quality, the MarqMetrix All-In-One Process Raman Analyzer delivers real-time monitoring of proteins, excipients, and other critical quality attributes. By enabling continuous in-line analysis, it reduces reliance on offline assays, shortens feedback cycles, and ensures product purity, potency, and consistency throughout purification and fill-finish workflows. This accelerates regulatory alignment and strengthens overall quality by design (QbD) strategies.

Technology and Design Advantages

• Inline analysis powered by Raman spectroscopy
• Compact footprint – Fits easily into laboratory or production environments.
• No recalibration required – End users never need to perform hardware calibration.
• Plug-and-play simplicity. With single-button hardware operation and intuitive Raman Suite software, anyone can generate reliable results without specialized training.
• Industry-leading optics – High-quality probe design ensures accurate, reproducible measurements.

The MarqMetrix Advantage:

The MarqMetrix All-In-One Process Raman Analyzer empowers organizations to modernize their analytical workflows by bringing real-time, in-line monitoring to every stage of bioprocess manufacturing. Whether in upstream cell culture or downstream purification, teams gain:
• Continuous process visibility to reduce variability and risk.
• Faster decision-making through immediate, high-quality data.
• Stronger compliance with FDA and EMA PAT guidelines.
• Improved efficiency and yield from automated feedback control.
• Reduced cost and complexity by eliminating unnecessary offline testing.
By delivering end-to-end bioprocess insight, the the MarqMetrix All-In-One Process Raman Analyzer supports the adoption of PAT and QbD approaches, helping biopharma companies improve process understanding, enhance product quality, and accelerate time-to-market.