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Biomarkers – Multimedia

Effective Fishing of Characteristic Proteome Fractions and Identification of Biomarkers Therein: Application of VisualCockpit to Multidimensional Chromatogram and MS Data content piece image
Poster

Effective Fishing of Characteristic Proteome Fractions and Identification of Biomarkers Therein: Application of VisualCockpit to Multidimensional Chromatogram and MS Data

Characteristic 2D fractions are rapidly identified among thousands using interactive visualization, filtering and data mining with VisualCockpit. Biomarker candidates are found therein after identifying proteins from sequence tags. VisualCockpit correlates their concentrations, reflected by normalized MS peptide peak height sum, enzyme activity and immunoreactivity, to donor conditions.
High-Throughput and High-Yield Purification of Recombinant Proteins Expressed in Escherichia coli. content piece image
Poster

High-Throughput and High-Yield Purification of Recombinant Proteins Expressed in Escherichia coli.

We have developed a high throughput expression of recombinant proteins containing hexahistidine affinity tag in Escherichia coli followed by one step affinity purification of proteins on Ni2+ agarose beads.
New Proteomic Subfractionation Surfaces – Innovative Technology for the Improved Resolution of Serum Proteins content piece image
Poster

New Proteomic Subfractionation Surfaces – Innovative Technology for the Improved Resolution of Serum Proteins

The data presented herein demonstrate the unique profiling capabilities of each ProFACT™ surface library subfraction and the collective resolution of 69 non-redundant proteins, calibrated and quantified from image analysis of SDS-PAGE profiles.
A Collaborative Approach to Developing Proteomics Data Standards content piece image
Poster

A Collaborative Approach to Developing Proteomics Data Standards

This poster provides an overview of the data standards work initiated between several parties, our current work, and the plans towards showcasing an example of a set of proteomics data standards at work in a large scale clinical proteomics setting, in a biomarker discovery context.
Metabonomics for MolPAGE Discovering Diabetes Biomarkers content piece image
Poster

Metabonomics for MolPAGE Discovering Diabetes Biomarkers

MolPAGE (Molecular Phenotyping to Accelerate Genomic Epidemiology) is an EU consortium with almost twenty collaborating universities and companies throughout Europe. One of the aims of MolPAGE is to find early onset biomarkersfor type 2 diabetes (T2DM) and cardio-vascular diseases(CVD).
IGF1 Biomarker Assay Validation content piece image
Poster

IGF1 Biomarker Assay Validation

This work demonstrates the development and validation of a novel clinical proteomics approach in which specific protein targets can be expeditiously and selectively isolated from acomplex biological fluid for MS characterization.
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