Gas Chromatography – Multimedia

Analysis of Herbal powders or ‘churnas’ using an optimized GC-MS/MS method is described. As they have high amount of matrix co-extractives, they pose critical analytical problems. The purpose of this work was overcome the effect of the matrix and develop a reliable method.

Analysis of dithiocarbamate pesticides (DTCs) using an optimized GC-MS method is described. DTCs are not stable and cannot be extracted or analyzed directly. The purpose of this work was develop a reliable method for identification and quantification of residues.

DBPs were identified in finished water, tap water, swimming pool water, and hot tub water that were not present in the source water. High Resolution Deconvolution was used effectively to identify a greater number of peaks in the chromatogram than by nominal resolution MS.

The Pegasus series of mass spectrometers are indispensable tools for metabolomics research:
-Throughput
-Discovery
-Confirmation
GC-HRT analyses resulted in confident metabolite identifications:
-Spectral similarity searches
-Formulae for fragment, molecular, and adduct ions

The data summarized in this presentation dispels myths associated with the utilization of GC-MS for analytes which are critical for metabolomic studies. GC-HRT provides high quality, accurate mass data for:
-Spectral similarity searches (NIST, Wiley, etc.)
-Formula determination (fragment, molecular & adduct ions)
-GC-HRT is an indispensible tool for the analysis of complex biological samples.

High Resolution GC-TOFMS and GC×GC-TOFMS are useful for a non-target comprehensive characterization of petroleum through a petroleomic approach, and can also provide targeted analysis of chemical markers.

High Resolution GC-TOFMS analysis with high resolution and high mass accuracy is crucial for identification of crude oil and asphaltene pyrolysis products by unequivocal chemical formula assignment, with enhancement of sensitivity and selectivity.

Proper sample preparation is critical for the analysis of complex samples. GC-HRT provides high quality, accurate mass data for:
-Spectral similarity searches (NIST, Wiley, etc.)
-Formula determination (fragment, molecular, and adduct ions)

Analyses of the M. truncatula extracts were performed, and data processing focused on qualitative analyses. This provided the opportunity for known analyte verification and unknown identification in a meaningful matrix and the demonstration of the key attributes and benefits of GC-HRT.

High resolution GC-TOFMS on the Pegasus GC-HRT allowed for quick and easy non-targeted screening of extractables from a common consumer product: the ubiquitous cell phone case. Most components could be identified with high similarity scores against commercial libraries. However, even when faced with low similarity scores against commercial libraries, such as in the case of Biphenol AF, the ability to rely on high mass accuracy allowed for confident identification of components.

GC-HRT methods utilizing hydrogen carrier gas were developed for analysis of representative specialty chemical, forensic, and metabolomic markets.