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Immunoassays – Multimedia

Transcreener™ KINASE Fluorescence Polarization Assay Performed on the PHERAstar content piece image
Poster

Transcreener™ KINASE Fluorescence Polarization Assay Performed on the PHERAstar

Transcreener™ KINASE ASSAY kits are far-red competitive fluorescence polarization immunoassays based on the detection of ADP. Any enzymatic reaction that uses ATP in a range from 1-250 µM can be monitored. With its dual emission detection and five photomultiplier tubes, BMG LABTECH´s PHERAstar provides the speed and sensitivity needed to take full advantage of BellBrook Labs Transcreener™ technology. Furthermore, a specific optical module was developed, thereby making assay setup simple.
Transcreener™ PDE Assay: Homogenous AMP and GMP Detection content piece image
Poster

Transcreener™ PDE Assay: Homogenous AMP and GMP Detection

To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP. The Transcreener™ PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies.
Transcreener™ PDE Assay: Homogenous AMP and GMP Detection content piece image
Poster

Transcreener™ PDE Assay: Homogenous AMP and GMP Detection

To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP. The Transcreener™ PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies.
A Novel Array- Based Assay for the Detection of Ig G-Mediated Food Intolerance content piece image
Poster

A Novel Array- Based Assay for the Detection of Ig G-Mediated Food Intolerance

We have developed a microarray based immunoassay to permit both greater food panel diversity and higher throughput testing. The Genarrayt™ 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner.
LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines content piece image
Poster

LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines

The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.
Quantitative Evaluation of High-Abundance Serum Proteins Depletion  content piece image
Poster

Quantitative Evaluation of High-Abundance Serum Proteins Depletion

Identification of low abundant proteins requires relative enrichment. We have tested systems for specific removal of high abundance proteins. Efficiency of albumin and IgG removal was assessed by ELISA and 2-DE. To estimate unspecific binding, six cytokines were spiked into serum. Recovery rates were determined with a cytometric bead assay.
A Novel Array-Based Assay for the Detection of IgG-Mediated Food Intolerance  content piece image
Poster

A Novel Array-Based Assay for the Detection of IgG-Mediated Food Intolerance

The Genarrayt 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner. Fluorescence intensity is directly proportional to antibody activity in the sample.
Combined Immune Parameters and X-ray data in Early Prediction of Anti-Tuberculosis Chemotherapy Response content piece image
Poster

Combined Immune Parameters and X-ray data in Early Prediction of Anti-Tuberculosis Chemotherapy Response

20 tuberculosis (12 slow-responders and 8 fast responders) patients were treated with directly observed short course anti-tuberculosis chemotherapy. Chest X-ray was performed. sICAM-1 and suPAR were measured in serum by ELISA, TNFRs using the luminex technology. General discrimination analysis on selected analytes gave, 91.66% and 87,50% correctly classify fast responders and slow responder respectively. The support vector machine analysis gave 100% correct classification.
IGF1 Biomarker Assay Validation content piece image
Poster

IGF1 Biomarker Assay Validation

This work demonstrates the development and validation of a novel clinical proteomics approach in which specific protein targets can be expeditiously and selectively isolated from acomplex biological fluid for MS characterization.
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