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The Immune System – Multimedia

Monitoring Dynamic Interactions of Tumor Cells with Tissue and Immune Cells in a Lab-on-a-Chip content piece image
Video

Monitoring Dynamic Interactions of Tumor Cells with Tissue and Immune Cells in a Lab-on-a-Chip

A complementary cell analysis method has been developed to assess the dynamic interactions of tumor cells with resident tissue and immune cells using optical light scattering and impedance spectroscopy to shed light on tumor cell behavior.
Reporter Bioassays to Assess Therapeutic Antibodies for Immunotherapy Programs content piece image
Poster

Reporter Bioassays to Assess Therapeutic Antibodies for Immunotherapy Programs

Immunotherapy, also called biologic therapy or biotherapy, stimulates certain parts of the immune system to fight diseases such as cancer. Important drug targets in immunotherapy include: Co-inhibitory receptors, such as PD-1/PD-L1, CTLA-4, LAG3, Tim3; and co-stimulatory receptors, such as GITR, CD40, OX40, 4-1BB.
Current approaches to assaying these targets are cumbersome and variable. Here we offer an improved in vitro bioassay approach.
Development of a Robust Reporter-based T cell Activation Assay for Therapeutic Biologics in Immunotherapy  content piece image
Poster

Development of a Robust Reporter-based T cell Activation Assay for Therapeutic Biologics in Immunotherapy

Jurkat T-cells stably expressing luciferase reporter driven by IL2 promoter or NFAT-RE, are used as effector cells. Tumor cell lines endogenously expressing cancer antigen are used as antigen presenting cells (APC). By co-cultivating the two cell lines in the presence of CD3 bispecific antibody, TCR/CD3 is activated in Jurkat effector cells. Luciferase activity is up regulated through IL-2 promoter or NFAT-RE activation.
Scaffold Design, Function and Over-expression of Lentiviral-based microRNAs content piece image
Poster

Scaffold Design, Function and Over-expression of Lentiviral-based microRNAs

Here we describe the strategy for scaffold design, the importance of an optimal promoter, and demonstrate gene target down-regulation from the over-expression of lentiviral microRNA mimics.
App Note / Case Study

Investigation of Cell Migration using a High Density Cell Exclusion Assay and Automated Microplate Imager

This application note investigates the use of high density cell exclusion assays for examination of cell migration.
Video

Highly-integrated Lab-on-a-chip System for Multiparameter Analysis at the Point-of-care

A lab-on-a-chip system called the "Fraunhofer ivD-platform" has been established which opens up the possibility for an on-site analysis at low costs.
Innovative Technology that Enables RNAi in Difficult to Transfect Cells content piece image
Poster

Innovative Technology that Enables RNAi in Difficult to Transfect Cells

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
Innovative Technology that Enables RNAi in Difficult to Transfect Cells content piece image
Poster

Innovative Technology that Enables RNAi in Difficult to Transfect Cells

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
Modelling CLL cell and T-cell Migration in a Dynamic Circulating Model of CLL content piece image
Poster

Modelling CLL cell and T-cell Migration in a Dynamic Circulating Model of CLL

We have recently developed a novel circulating model of chronic lymphocytic leukaemia (CLL) that mimics the transient interactions that take place between circulating lymphocytes and vascular endothelium. Here we show that both normal and malignant lymphocytes actively underwent transendothelial migration.
Quantification of Natural Killer Cell-Mediated Cytotixicity using Celigo Imaging Cytometry content piece image
Poster

Quantification of Natural Killer Cell-Mediated Cytotixicity using Celigo Imaging Cytometry

Time-course tracking of % lysis eliminates multiple controls & the effect of non-uniform cell seeding in the final cytotoxicity calculation. The # of cells used is less than the cells needed for Release assays & Flow Cytometry. Flow cytometry & Release assays require a seeding density of 100K target cells increasing the number of effector cells to the millions. The visual observation of ADCC or CDC on the images can be convincing to conclude the functionality of antibodies or complements.
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