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The Immune System – Multimedia

App Note / Case Study

Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
Comparison of Three Methods for the Evaluation of Cytokine Storm Risk in Early and Clinical Stage Biopharmaceutical Development content piece image
Poster

Comparison of Three Methods for the Evaluation of Cytokine Storm Risk in Early and Clinical Stage Biopharmaceutical Development

Objective: To identify an assay that can accurately predict the risk of CRS and CS associated with investigational biotherapeutics. A comparison of three methods were used: (a) immobilization of test antibody on plastic, (b) co-culture of PBMC's on HUVEC's and (c) pre-culture of PBMC's at high cell density.
App Note / Case Study

Efficient washing of magnetic beads

The use of magnetic beads as the solid phase in a multiplexed ELISA enables high throughput cytokine screening and offers several advantages over common ELISA assays with direct coupling of the analyte to the well-surface.
Video

DNA Methylation and the Cancer Epigenome Biological and Translations Implications

In this talk, Stephen Baylin, discusses the development of strategies for “epigenetic therapies” for cancer and biomarker development.
Does the increase of exosomal microRNAs reflect an activated immune system in melanoma? content piece image
Poster

Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?

Cutaneous malignant melanoma is a form of skin cancer that accounts for 65% of skin cancer-related deaths. The incidence increases continuously, and while early detection leads to nearly 100% survival rates, the mortality raises to greater than 80% for patients with advanced disease.
Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies content piece image
Poster

Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
Molecular Dynamics Simulation Study of Pulmonary Surfactant Interacting With Nanoparticles content piece image
Poster

Molecular Dynamics Simulation Study of Pulmonary Surfactant Interacting With Nanoparticles

MD simulation studies using NAMD of lipid bilayers supported on alpha-quartz (nanoparticles) and kaolinite with explicit water molecules will be presented to understand the physiochemical effects of nanoparticles on pulmonary surfactant.
Hypothesis of an Existence of a Reverse Pathway (Rp) which Passes Genetic Information from Polypeptide Antigens to Ig Genes in B-Lymphocytes content piece image
Poster

Hypothesis of an Existence of a Reverse Pathway (Rp) which Passes Genetic Information from Polypeptide Antigens to Ig Genes in B-Lymphocytes

Results of this research will bring new more effective treatment methods of many immune related diseases, including cancer.
Molecular Dynamics Simulation Study of Pulmonary Surfactant Interacting With Nanoparticles content piece image
Poster

Molecular Dynamics Simulation Study of Pulmonary Surfactant Interacting With Nanoparticles

MD simulation studies using NAMD of lipid bilayers supported on alpha-quartz (nanoparticles) and kaolinite with explicit water molecules will be presented to understand the physiochemical effects of nanoparticles on pulmonary surfactant.
App Note / Case Study

Direct Visualisation, Sizing & Counting of Viruses and Phages in Liquid Suspension

A new laser-based nanoparticle tracking analysis system is now available which allows nanoscale particles such as viruses and virus aggregates to be directly and individually visualised in liquids in real-time, from which high-resolution particle size distribution profiles can be obtained.
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