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The Immune System – Multimedia

App Note / Case Study

Automated Liquid-liquid Extraction of 25-Hydroxy Vitamin D from Serum and Preparation for LC/MC Analysis Using the Hamilton Robotics Microlab STARlet Workstation

This application note demonstrates the automation capabilities of Hamilton Robotics’ Microlab STARlet and how it can be used to prepare clinical serum samples for Vitamin D analysis quickly, reliably and cost effectively.
Development of an Automated Platform for HT Cloning and Expression content piece image
Poster

Development of an Automated Platform for HT Cloning and Expression

Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.
Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility content piece image
Poster

Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility

Since the end of the 1990’s, the pharmaceutical industry has seen an increased interest in biologics, especially in the therapeutic areas of oncology and inflammation. Here we present the automation of two assays for the characterization and selection of potent antibody drug candidates. Both assays rely on HTRF® detection. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells.
App Note / Case Study

ESGRO-2i Medium: Inhibitor-Based Serum-free Medium for ES and iPS Cell Culture

Defined serum-free and feeder-free culture of mouse embryonic stem (mES) cells holds many advantages over the classical serum-containing feeder-dependent culture methods, ranging from decreased lot-to-lot variations to ease of culture.
Antigen Determination in Autoimmune Hepatitis Type1 content piece image
Poster

Antigen Determination in Autoimmune Hepatitis Type1

Objectives of this project were to exploit the database in indian setting to determine nuclear antigens as target for antinuclear antibodies (ANA) in patients of autoimmune hapatitis (AIH) type1.
Quantification of cytokines on the SpectraMax® Paradigm® Multi-Mode Microplate Detection Platform using Alpha Technology content piece image
Poster

Quantification of cytokines on the SpectraMax® Paradigm® Multi-Mode Microplate Detection Platform using Alpha Technology

Inflammation is accompanied by increased endothelial chemokine production and adhesion molecule expression, which may result in an extensive neutrophil infiltration. As such, the search for novel anti-inflammatory substances able to downregulate these parameters, as well as tissue damage, holds therapeutic promise.
Identification of novel autoantigensin patients with liver autoimmune diseases by Protein MicroArray content piece image
Poster

Identification of novel autoantigensin patients with liver autoimmune diseases by Protein MicroArray

The characterization of autoimmune disease-specific biomarkers are of primary importance for the development of diagnostic tools and the comprehension of pathogenetic mechanisms leading to autoimmunity. To this aim a protein microarray was employed to analyze serum samples from patients with autoimmune hepatitis (e.g. AIH & PBC) and of healthy as controls. A panel of autoantigens able to discriminate among the groups of patients was identified for potential use as biomarkers.
Neutrophil Adhesion: A HCS Compatible Assay Using the Acumen eX3 content piece image
Poster

Neutrophil Adhesion: A HCS Compatible Assay Using the Acumen eX3

The Acumen eX3 can be used to study the process of cellular adhesion, whereby adherent cells types specifically, endothelial cells can be grown to confluence in microtitre plate wells and other cells types e.g. neutrophils added. The neutrophils can be differentially stained with calcein AM and the adhesion profile monitored and quantified. Cell adhesion can be determined simply by correlating retained fluorescence with cell number.
New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells content piece image
Poster

New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells

We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue
Sub-classification of Colorectal Cancer Using Surface Antigen Antibody Microarray and Fluorescence Multiplexing content piece image
Poster

Sub-classification of Colorectal Cancer Using Surface Antigen Antibody Microarray and Fluorescence Multiplexing

Colorectal cancer (CRC) is the second most frequent cause of cancer deaths in Australia. Even after resection up to 50% of patients relapse. In an attempt to prevent recurrences chemotherapy is administered to high risk patients. However, as few as 10-20% patients genuinely benefit because the clinical course for individuals with CRC remains difficult to predict, largely due to prognostically heterogeneous groups within same-stage tumour categories.
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