Biopharma 101: Analysis of CRISPR Gene Editing Systems

The CRISPR/Cas system has been increasingly used as a genome editing tool with applications that include disease treatment. The safety and efficacy of these genetic medicines depend on the stability and purity of each molecular component, which can be assessed and characterized by numerous analytical techniques.

Biopharma 101 is intended to give you an introduction to analytical workflows for specific therapeutic modalities. In this webinar, our expert speakers will focus on various gene editing systems and how you can utilize analytical instrumentation to characterize the various components. 

• Characterize an engineered Cas9 protein, its amino acid mutations and post-translational modifications using LC-MS/MS 
• Evaluate purity and sizing of CRISPR systems with capillary gel electrophoresis (CGE) to overcome technical challenges and minimize impacts of higher-order structure
• Determine RNA integrity and purity, critical to ensuring the efficacy of RNA-based therapeutics and vaccines 
• Understand plasmid quality with capillary gel electrophoresis laser-induced fluorescence (CGE-LIF)